搜风祛痰中药对动脉粥样硬化ApoE基因敲除小鼠血脂及IL-1β的影响

Effect of Soufeng Qutan Chinese medicine on blood lipid and IL-1β in atherosclerotic mice with ApoE gene knockout

目的:探讨搜风祛痰中药治疗动脉粥样硬化的可能作用机制。方法:选取6周大ApoE基因敲除小鼠高脂饮食喂养13周建立动脉粥样硬化不稳定斑块模型,并随机分为模型组、阿托伐他汀组以及搜风祛痰低、中、高剂量组;另取6周大C57BL/6J小鼠设为空白对照组。空白对照组及模型组均给予0.9%生理盐水灌胃,搜风祛痰中药低、中、高剂量组以及阿托伐他汀组分别给予不同剂量的搜风祛痰中药汤剂和阿托伐他汀片进行干预。13周后处死小鼠,HE染色观察斑块面积,并利用生化自动分析仪检测血脂总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)含量。ELISA法检测IL-1β水平。结果:与空白对照组相比,模型组可见大量泡沫细胞,斑块面积明显;血清TC、TG、LDL-C、IL-1β表达水平均显著升高(P<0.05),HDL-C表达水平显著降低(P<0.05);与模型组相比,搜风祛痰中药各剂量组和阿托伐他汀组有少量泡沫细胞,斑块面积减少;血清TC、TG、LDL-C、IL-1β表达水平均显著降低(P<0.05),HDL-C表达水平显著升高(P<0.05)。结论:搜风祛痰中药可抑制细胞炎症反应的进展,下调IL-1β的表达,减少斑块脂质含量,改善血脂,这可能是搜风祛痰中药早期治疗动脉粥样硬化的分子机制之一。

Objective:To explore the possible mechanism of Soufeng Qutan traditional Chinese medicine in the treatment of atherosclerosis.Methods:Mice of six weeks old which were knocked out gene ApoE and fed with a high-fat diet for 13 weeks to establish a model of atherosclerotic unstable plaque,and they were randomly divided into the model group,the atorvastatin group and the low,medium,and high-dose Shoufeng Qutan groups.In addition,six weeks old C57BL/6J mice were set as the blank control group. Both the blank control group and the model group were given 0.9% normal saline by gavage. The low,medium,and high-dose groups of Soufeng Qutangroup and the atorvastatin group were given different doses of Soufeng Qutan Chinese Medicine Decoction and atorvastatin. After 13 weeks,the mice were sacrificed,and the abdominal aortic blood vessels were observed by HE staining. Total cholesterol(TC),triglycerides(TG),low-density lipoprotein cholesterol(LDL-C). and high-density lipoprotein cholesterol(HDL-C)content were detected by a biochemical automatic analyzer and IL-1β levels were detected by ELISA.Results:Compared with the blank control group,a large number of foam cells could be seen in the model group with obvious plaques;serum TC,TG,LDL-C,and IL-1β levels were significantly increased(P<0.05),and HDL-C level was markedly decreased(P<0.05). Compared with the model group,the Soufeng Qutangroup at all doses and the atorvastatin group had a small amount of foam cells and reduced plaque area;serum TC,TG,LDL-C,and IL-1β levels were significantly decreased(P<0.05),and the level of HDL-C was significantly increased(P<0.05).Conclusion:Soufeng Qutan Chinesemedicine can inhibit the progression of cellular inflammation,downregulate the expression of IL-1β,reduce plaques,and improve blood lipids. This may be one of the molecular mechanisms of Soufeng Qutan Chinese medicine in the early treatment of atherosclerosis.