中成药贯黄感冒颗粒抗人呼吸道合胞体病毒活性的研究

Study of Guanhuang Ganmao Keli on the antiviral activity on human respiratory syncytial virus

目的:研究治疗感冒用中药贯黄感冒颗粒体外抗人呼吸道合胞体病毒(human respiratory syncytial virus,RSV)活性。方法:将中药贯黄感冒颗粒溶解于纯水,经0.22微米滤器过滤后制成贯黄感冒颗粒水溶液。以环盐酸吗甲吡嗪酸(cyclopiazonic acid,CPA)为阳性对照药物,利用CCK-8法测定贯黄感冒颗粒水溶液对Hep-2细胞的细胞毒性。在对细胞最大无毒的浓度下,测定贯黄感冒颗粒对RSV的抗病毒效果。结果:贯黄感冒颗粒的半数致死浓度(TC_(50))为0.647 mg/mL,半数有效浓度(EC_(50))为0.014 mg/mL,治疗指数(TI)为46.21。贯黄感冒颗粒在RSV感染Hep-2细胞后0、2、4、6、8 h给药,结果表明贯黄感冒颗粒具有显著的抗RSV效果。结论:贯黄颗粒在体外培养细胞体系检测下对RSV具有明显的抑制作用。

Objective:To study the antiviral activity of Guanhuang Ganmao Keli on human respiratory syncytial virus(RSV)in vitro.Methods:The Guanhuang Ganmao Keli was dissolved in pure water and filtered by a 0.22 micron filter to get solution.Cyclopiazonic acid(CPA)was used as positive control.The toxicity of Guanhuang Ganmao Keli on Hep-2 cells was tested by cell counting kit 8(CCK-8).The protective effect of Guanhuang Ganmao Keli on RSV was evaluated under the highest toxic concentration.Results:The TC_(50) and EC_(50) of Guanhuang Ganmao Keli is 0.647 mg/mL and 0.014 mg/mL,respectively.Guanhuang Ganmao Keli showed significant antiviral effect when added 0、2、4、6 and 8 h post-infection.Conclusion:Guanhuang Ganmao Keli is an effective antiviral agent on RSV in vitro.