血必净对大鼠睾丸扭转/复位缺血再灌注损伤的修复作用

Repairing Effect of Xuebijing on Testicular Torsion/Detorsion-Induced Ischemia/Reperfusion Injury in Rats

【目的】探讨血必净注射液对大鼠睾丸扭转/复位缺血再灌注(I/R)损伤的修复作用。【方法】将40只雄性SD大鼠随机分为假手术组,模型组,血必净低、高剂量组和地塞米松组,每组8只。除假手术组外,其他各组大鼠构建睾丸扭转/复位模型。术后,血必净低、高剂量组和地塞米松组大鼠分别对应腹腔注射0.5、2.0 mL·kg^(-1)·d^(-1)血必净注射液和0.5 mL·kg^(-1)·d^(-1)地塞米松,连续3 d。给药结束后,采用苏木素-伊红(HE)染色法观察睾丸组织病理学改变,酶联免疫吸附分析(ELISA)检测血清肿瘤坏死因子α(TNF-α)、白细胞介素1β(IL-1β)和Toll样受体4(TLR4)的含量,采用硫代巴比妥酸法检测睾丸组织丙二醛(MDA)含量,采用羟胺法测定睾丸组织超氧化物歧化酶(SOD)水平,末端脱氧核苷酸转移酶介导的d UTP缺口末端标记(TUNEL)染色法检测睾丸组织生殖细胞凋亡水平。【结果】与假手术组比较,模型组睾丸曲细精管排列紊乱,生殖细胞脱落,睾丸组织MDA含量、生殖细胞凋亡水平,血清TNF-α、IL-1β和TLR4水平均升高,睾丸组织SOD水平降低(均P<0.05)。与模型组比较,血必净组生精细胞排列逐渐清晰,脱落细胞减少,睾丸组织MDA含量、生殖细胞凋亡水平,血清TNF-α、IL-1β和TLR4水平均降低,睾丸组织SOD水平升高(均P<0.05)。【结论】血必净可改善睾丸I/R病理损伤,降低生殖细胞的凋亡,减轻氧化应激,降低炎症因子的表达,对大鼠睾丸扭转/复位I/R损伤有修复作用。

Objective To exlpore the repairing effect of Xuebijing Injection on testicular torsion/detorsion-induced ischemia/reperfusion(I/R)injury in rats.Methods Forty male SD rats were randomly divided into sham operation group,model group,Xuebijing low-dose and high-dose groups,and Dexamethasone group,with 8 rats in each group.Except for the sham operation group,testicular torsion reduction model was constructed in other groups.After surgery,the rats in the low-dose and high-dose groups and Dexamethasone group were intraperitoneally injected with 0.5,2.0 m L·kg^(-1)·d^(-1)of Xuebijing Injection and 0.5 mL·kg^(-1)·d^(-1)of Dexamethasone,respectively,for 3 consecutive days.After drug administration,hematoxylin-eosin(HE)staining was used to observe the testicular histopathological changes,and the contents of tumor necrosis factorα(TNF-α),interleukin 1β(IL-1β)and Toll-like receptor 4(TLR4)in serum were detected by enzymolase-linked immunosorbent assay(ELISA).The content of malondialdehyde(MDA)in testicular tissue was measured by thiobarbituric acid assay,the level of superoxide dismutases(SOD)in testicular tissue was measured by hydroxylamine method,and the germ cell apoptosis level in testicular tissue was detected by terminal deoxynucleotidyl transferase of d UTP terminaldeoxynucleoitidyl transferase mediated nick end labeling(TUNEL)staining.Results Compared with the sham operation group,convoluted tubules of testis in the model group were disordered,germ cells were detached,MDA content in testicular tissue,germ cell apoptosis level,serum TNF-α,IL-1βand TLR4 levels were increased,and SOD level in testicular tissue was decreased(all P<0.05).Compared with the model group,spermatogenic cells in the Xuebijing group were arranged more clearly with decreased shed cells,MDA content and apoptosis level of germ cells in testicular tissue were decreased,serum TNF-α,IL-1βand TLR4 levels were decreased,and SOD level in testicular tissue was increased(all P<0.05).Conclusion Xuebijing is effective for improving the pathological injury of testicular I/R,apoptosis of germ cells,oxidative stress,and the expression of inflammatory factors,thereby playing a role in the repair of testicular testicular torsion/detorsion-induced I/R injury in rats.