金银花快繁体系构建及其总黄酮含量的测定

Study on Rapid Propagation of Lonicera japonica and Measurement of Total Flavonoids Content

为构建长沙野生金银花组织快繁体系,探讨金银花组织快繁不同材料中总黄酮含量变化,试验使用2,4-D、6-BA、NAA等不同激素配比的MS培养基进行组织培养,并用紫外可见分光光度计法检测总黄酮含量。结果表明:MS+2,4-D 4.0 mg/L为诱导愈伤组织的最适培养基配方,MS+6-BA 1.0 mg/L+NAA 0.10 mg/L为诱导不定芽的最适培养基配方,MS+6-BA 1.5 mg/L+NAA 0.05 mg/L为不定芽扩繁最适培养基配方,诱导生根的最适培养基配方为1/2MS+NAA 2.0 mg/L。愈伤组织中的总黄酮含量最高,其次是外植体茎叶,再生植株茎叶中含量最低。试验建立了长沙野生金银花组织快繁体系,得到了金银花组织快繁不同材料中总黄酮含量变化曲线。

This research aims to set up a rapid propagation system of Changsha wild Lonicera japonica,and to explore the change of total flavonoids content in various propagation materials of Lonicera japonica.MS media consisted of 2,4-D,6-BA and NAA were used for tissue culture of Lonicera japonica.And UV-Vis spectrophotometer assay was used to test total flavonoids content.Results demonstrated that MS+2,4-D 4.0 mg/L was the optimum medium for inducing calli.MS+6-BA 1.0mg/L +NAA 0.10 mg/L was the optimum medium for the adventitious buds induction.MS+6-BA 1.5mg/L+NAA 0.05mg/L was the optimum proliferation medium of adventitious buds.1/2MS+NAA 2.0mg /L was the optimum medium for roots regeneration.The total flavonoids content in the calli was the highest,followed by the stems and leaves of the explants,the least content was in the stems and leaves of the test-tube plantlets.This study has established a complete rapid propagation system of Lonicera japonica,and gained the change curve of total flavonoids in various propagation materials of Lonicera japonica.