黑杨派杨树种质资源的SSR分析及鉴定

Analysis and Identification of Section Aigeiros Germplasm Based on SSR Molecular Markers

以50份黑杨派种质资源为试验材料,利用筛选的15对SSR引物对其进行遗传多样性分析和鉴定,依据相似系数构建聚类图并进行遗传关系分析,结果表明:15对引物共检测到187个多态位点,多态性位点百分率为100%,每对引物检测到多态位点数8~19个,平均12.47个;期望杂合度(H_(e))平均为0.820 7,观测杂合度(H_(o))平均为0.739 1,多态信息含量(PIC)平均为0.79;有12对引物在17份样品中检测到了特异性位点,共检测到特异性位点36个,每个引物检测到特异性位点数量为1~5个。UPGMA聚类分析表明,50份杨树种质相似系数为0.727 3~0.989 3,平均0.804 5,具有较高的遗传相似性,当相似系数在0.79时,参试样品可分为4大类,聚类分析结果与种质的系谱关系及引种种源基本吻合,能够较好的反映种质间的亲缘关系;综合特异性位点的检测和聚类分析的结果,初步判断N024、N018、N017、N009、YN和47#可能不是纯种美洲黑杨,N4和N51可能不是纯种欧洲黑杨,还有待于进一步验证。依据各引物等位位点特征,选取引物组合GCPM-2826、P139、P3和P141构建辽宁地区主栽的14份种质的指纹图谱,能够有效的区分14份种质。

The genetic diversity analysis and identification were carried out on 50 section Aigeiros germplasm with 15 pairs of SSR primers screened,and the dendrogram was constructed to conduct genetic relationship analysis based on genetic similarity coefficients.The results showed that a total of 187 polymorphic loci were detected with 15 pairs of primers,and the percentage of polymorphic loci was 100%.The number of polymorphic loci detected by each pair of primers ranged from 8 to 19 with an average of 12.47.The average expected heterozygosity(H_(e)) was 0.820 7,and the observed heterozygosity(H_(o)) averaged 0.7391.The average polymorphism information content(PIC) was 0.79.Among them,12 pairs of primers detected specific sites in 17 samples,a total of36 specific sites were detected,and the number of specific sites was detected by each pair of primers ranged from1 to 5.UPGMA cluster analysis was performed on 50 poplar germplasm.The genetic similarity coefficient ranged from 0.727 3 to 0.989 3 with an average of 0.804 5,have high genetic similarity.When the similarity coefficien was 0.79,the samples could be divided into 4 categories,and the cluster analysis results were consistent with the genealogical relationship and introduction provenance of germplasm,which could better reflect the genetic relationship between germplasm.Based on the results of specific site detection and cluster analysis,it was preliminarily determined that N024,N018,N017,N009,YN and 47#may not be pure P.deltoides,and that N4 and N51may not be pure P.nigra,which needs further verification.According to the allelic site characteristics of each primer,the primer combinations GCPM-2 826,P139,P3 and P141 were selected to construct the fingerprint of 14germplasm,which could effectively distinguish 14 germplasm.