银杏类黄酮代谢研究进展

Advances in Flavonoid Metabolism of Ginkgo biloba

基于类黄酮化合物在银杏叶用林培育、药物生产加工等方面的重要理论研究和应用价值,从环境、细胞培养、分子水平3个方面对近年来银杏类黄酮代谢的调控研究进行了综述。得出光照、温度、水分等环境条件的改变可以调控银杏类黄酮的代谢;诱导子、微量元素的调整是细胞培养中类黄酮生产的有效调控手段;随着银杏全基因组测序地完成,人们通过基因组学与高通量测序技术挖掘出了许多关键酶基因和转录因子,丰富了人们对银杏黄酮代谢进行调控的手段。在未来的应用和研究中,建议通过多组学研究挖掘更多的相关基因和关键酶,从上游和下游的基因簇共调节研究黄酮的代谢通路,并结合合成生物学实现银杏黄酮的智能调控。

Based on the values of flavonoids research and application in Ginkgo biloba forest cultivation,drug production, we reviewed recent progress of flavonoids metabolic regulation in environment, cell culture and molecular level in this paper. It was concluded that the changes of light, temperature, water and other environmental conditions could regulate the metabolism of G. biloba flavonoids. The induction of elicitors and trace elements was effective method to regulate the production of flavonoids in cell culture. With the complete genome sequencing of G. biloba, many key enzyme genes and transcription factors have been discovered through genomics and high-throughput sequencing technology, and this enriched the control ways of the flavonoids metabolism in G. biloba. In the future, more related genes and key enzymes should be excavated through multi-omics research,and it is necessary to research the metabolic pathway of flavonoids by using upstream and downstream of gene clusters combined regulation, and to intelligently regulate of G. biloba flavone synthesis by combining with synthetic biology.