光电关联显微镜技术及其在植物学研究中的应用

Correlative Light and Electron Microscopy and Its Application in Botanical Research

光电关联显微镜技术(correlative light and electron microscopy,CLEM)将光学显微镜的高灵敏度和大视场与电子显微镜的高分辨率相结合,弥补了各自成像的局限,可在原位获得更全面、更精细的定位及结构信息,近年来受到广泛关注。目前,该技术广泛应用于亚细胞结构与特定结构的观察、蛋白质的精确定位、囊泡运输及植物免疫等多个重要研究领域。本文总结了CLEM的概念和原理,对标记探针进行了总结分类,着重就该技术在植物学领域的应用以及存在的问题进行了讨论,并对该技术未来的发展与应用作出了展望。

Correlative light and electron microscopy(CLEM)combines the high sensitivity and wide view of light microscopy with the excellent spatial resolution of the electron microscopy,and it overcomes the limitations of their respective imaging,enabling to obtain more comprehensive and fine positioning and structural information in situ,thus widespread attention has been paid to it in recent years.As a powerful attractive technique,CLEM has been widely used in the observation of subcellular structures and specific structures,precise localization of proteins,and the study of vesicle transport and plant immunity.Here,we summarize the concept and principle of CLEM and sum up the probes used in CLEM.Then we discuss the application of CLEM in the field of botany and point out the existing issues.Finally,we propose some aspects for the future development and application of CLEM.