不同提取工艺对清肺散有效成分提取效率的影响

Effects of Different Extraction Processes on the Contents of Active Ingredients in Qingfei Powder

为筛选清肺散的理想提取方法,试验采用生物发酵法、酶提法和水煎法三种中药提取工艺,以(R,S)-告依春、甘草苷、甘草酸铵和槲皮素-3-O-β-D-葡萄糖-7-O-β-D-龙胆双糖苷为考核指标,应用液相色谱法测定不同方法提取清肺散有效活性成分的效果。结果显示,在相同物理条件下,生物发酵法对清肺散有效成分提取量分别均高于纤维素酶提取法和水煎提取法,3种方法之间清肺散有效成分含量提取率存在着极显著差异(P<0.01)。试验表明,生物发酵法中药有效成分的提取效果最优,其次是纤维素酶提取法和水煎法,研究结果为清肺散活性成分的提取提供了技术参考。

In order to screen the ideal extraction method of Qingfei powder,the experiment adopted three traditional Chinese medicine extraction processes:biological fermentation,enzymatic extraction and water decoction,with Epigoitrin,ammonium glycyrrhizinate and quercetin-3-O-β-D-glucose-7-O-β-D-gentiobiglycoside as the assessment indexes,and liquid chromatography was used to determine the effect of different methods of extracting effective active ingredients of Qingfei powder.The results showed that under the same physical conditions,the extraction amount of the effective ingredients of the drug by the biological fermentation method was higher than that of the cellulase extraction method and the water decoction extraction method,respectively.There was a significant difference in the extraction rate of the effective ingredient content of Qingfei powder among the three methods(P<0.001).The results showed that the microbial fermentation method had the best extraction effect on the active components of Qingfei powder,followed by cellulase extraction and water decoction.This study would provide a technical reference for the extraction of the active ingredients of traditional Chinese medicine.