微波辅助提取辣木籽总苷工艺优化及其抗氧化活性研究

Optimization of Microwave-assisted Extraction of Total Glycosides from Moringa oleifera Seeds and Its Antioxidant Activity

为有效利用和开发辣木籽,以乙醇为溶剂,采用微波辅助法提取辣木籽中的苷类物质,以辣木籽总苷提取量为评价指标,在单因素试验的基础上,运用响应面优化提取工艺,采用水杨酸法、ABTS法、FRAP法测定优化后辣木籽提取物各极性部位体外抗氧化活性。结果表明,响应面分析法得到的最佳提取条件为:乙醇体积分数81%,料液比1:30 g/mL,提取时间20 min,微波功率600 W;体外抗氧化活性顺序为:乙酸乙酯部位>石油醚部位>正丁醇部位>水部位。由此表明,用微波辅助法提取辣木籽中的苷类物质,响应面法优化辣木籽总苷提取工艺,方法可行。体外抗氧化活性试验表明,辣木籽醇提物各极性部位对自由基具有清除能力,可为进一步开发天然抗氧化剂奠定基础。

In order to effectively utilize and develop Moringa oleifera seeds,the glycosides in Moringa oleifera seeds were extracted by microwave-assisted method with ethanol as solvent.Based on the single factor experiment,the extraction process was optimized by response surface methodology.The antioxidant activities in vitro of each polar part of the optimized Moringa oleifera seeds extract were determined by salicylic acid method,ABTS method and FRAP method.The results showed that the optimal extraction conditions were as follows:Ethanol volume fraction 81%,solid-liquid ratio 1:30 g/mL,extraction time 20 min,microwave power 600 W.The order of antioxidant activity in vitro was ethyl acetate>petroleum ether>n-butanol>water.The results indicated that the microwave-assisted extraction of glycosides from Moringa oleifera seeds and the optimization of the extraction process of total glycosides from Moringa oleifera seeds by response surface methodology was feasible.In vitro,antioxidant activity tests showed that the polar parts of ethanol extracts of Moringa oleifera seeds had free radical scavenging ability,which laid a foundation for the further development of natural antioxidants.