Rapid Screening of 82 Pesticide Residues in Feed by Liquid Chromatography Coupled with Quadrupole-Time of Flight Mass Spectrometry

[Objectives]This study was conducted to establish a qualitative method for rapid detection of pesticide residues in feed.[Methods]A rapid screening method for 82 pesticide residues in feed was established using ultra liquid chromatography coupled with quadrupole-time of flight mass spectrometry(UPLC-Q-TOF-MS)combined with optimized pretreatment methods.[Results]Feed samples were extracted with 0.2%formic acid-acetonitrile solution,shaken,centrifuged,and re-centrifuged,and the supernatant was filtered with a 0.22μm filter membrane for determination.The samples were separated on a Waters ACQUITY UPLC HSS T3 chromatographic column(2.1 mm×50 mm,1.8μm),and subjected to gradient elution with methanol-water solution as the mobile phase,and scanning was performed in ESI+mode.82 compounds could be accurately qualitatively detected in 13 min.[Conclusions]This method is simple to operate,time-saving,and can be applied to rapid screening of large-scale feed.This study provides a theoretical basis for the development of new methods in the future.