不同光质对红花檵木愈伤组织生长及黄酮类物质含量的影响

Effect of Different Light Quality on Callus Growth and Flavonoids Content of Two Loropetalum chinense Plants

该试验以红花檵木叶片诱导的愈伤组织为试验材料,以黑暗(CK)为对照,分别进行白光(W)、红光(R)、蓝光(B)、蓝紫光(BP)、蓝光+UV-A(B+UV-A)和UV-A处理15 d和30 d,通过对愈伤组织的生长、生理指标、花色素苷和总黄酮含量的比较分析,探讨光质对红花檵木愈伤组织生长和黄酮类物质含量的影响。结果显示:(1)与CK相比,几种不同光质处理均可提高愈伤组织的干、鲜重,其中R更有利于愈伤组织鲜重的增加,B+UV-A和UV-A更有利于干重的增加,而且光质对愈伤组织干重的影响存在基因型差异。(2)R、B和W处理可显著提高红花檵木愈伤组织的可溶性糖含量,其中R处理的增加最高,B和W处理其次,其他短波处理与0 d相比稍变化。(3)短波长的光质处理显著提高了红花檵木愈伤组织中的丙二醛(MDA)含量,其中B+UV-A处理的增值最高。(4)短波长的光质更有利于提高红花檵木愈伤组织的苯丙氨酸解氨酶(PAL)活性,其中BP和B+UV-A处理的增效最为显著。(5)短波长的光质(B、BP、B+UV-A和UV-A)可促进红花檵木愈伤组织花色素苷和黄酮类物质的合成,但R处理具有抑制作用。研究表明,短波光质B、BP、B+UV-A和UV-A处理可促进红花檵木愈伤组织花色素苷合成,B+UV-A和B处理更有利于总黄酮含量的提高,而且在愈伤组织干重、花色素苷与总黄酮含量方面光质的影响存在一定的基因型差异。

In order to clarify the influence mechanism of light quality on the growth of Loropetalum chinense var.rubrum callus and the synthesis and accumulation of secondary metabolites flavonoids,to provide a theoretical basis for man-made regulation of the yield and quality of its cell products,we used,the callus induced by L.chinense var.rubrum leaves as the test material in this experiment with the darkness(CK)as control.White light(W),red light(R),blue light(B),blue-violet light(BP),and blue light+UV-A(B+UV-A)and UV-A treat for 15 and 30 d,respectively.Through comparative analysis of callus growth,physiological indicators,anthocyanin and total flavonoids content,we explored the effect of light quality on L.chinense var.rubrum callus growth and flavonoid content.The results show that:(1)compared with CK,several different light quality treatments can increase the dry and fresh weight of callus.Among them,R is more conducive to the increase of callus fresh weight,B+UV-A and UV-A are more conducive to the increase of dry weight,and there are some genotypic differences in the influence of light quality on the dry weight of callus.(2)R,B and W treatments can significantly increase the solubility of L.chinense var.rubrum callus sugar content,among which R treatment has the highest value-added,B and W treatments second,and other short-wave treatments slightly increase or decrease compared with 0 d.(3)Short-wavelength light quality treatment significantly improves the callus of L.chinense var.rubrum.Among them,B+UV-A treatment has the highest value-added content.(4)Short-wavelength light quality treatment is more conducive to increasing the PAL activity of L.chinense var.rubrum callus.Among them,BP and B+UV-A treatments are the most significant.(5)Short-wavelength light quality(B,BP,B+UV-A and UV-A)can promote the synthesis of anthocyanins and flavonoids in the callus of L.chinense var.rubrum,while R treatment play an inhibitory role.It was shown that short-wave light quality B,BP,B+UV-A and UV-A treatments can promote the synthesis of anthocyanins in the callus of L.chinense,B+UV-A and B treatments are more conducive to the increase of total flavonoids content;Moreover,there are certain genotypic differences in the influence of light quality on the dry weight of callus,anthocyanin and total flavonoids.