长链非编码RNA SNHG1在子宫内膜癌中的表达及调控PI3K/AKT信号通路的研究

Expression of Long Non-coding RNA SNHG1 in Endometrial Carcinoma and Its Regulation of PI3K/AKT Signaling Pathway

目的研究长链非编码RNA小核仁RNA宿主基因1(lncRNA SNHG1)在子宫内膜癌组织中的表达及临床意义,并探讨SNHG1在子宫内膜癌中的生物学功能。方法应用实时荧光定量PCR(qRT-PCR)检测SNHG1在54例子宫内膜癌组织和癌旁组织中的表达水平,并分析子宫内膜癌组织中SNHG1的表达水平与患者临床病理参数的关系以及其对子宫内膜癌患者预后的影响。SNHG1小干扰RNA(siRNA)转染子宫内膜癌细胞系Ishikawa,分为si-NC组和si-SNHG1组,应用qRT-PCR检测SNHG1 siRNA转染效果。干扰SNHG1的表达后,采用MTS实验检测细胞的增殖活性,流式细胞仪检测细胞周期情况,Transwell实验检测细胞的转移能力,western blotting检测细胞中PI3K/AKT信号通路激活情况,Logrank法比较SNHG1高低表达人群的生存率差异。结果SNHG1在子宫内膜癌组织中的表达高于癌旁组织(t=5.236,P=0.000),其高表达与FLGO分期、肌层浸润深度和淋巴结转移有关(t=2.289~3.136,P=0.003~0.037),且高表达SNHG1的子宫内膜癌患者生存率较低(χ^(2)=33.266,P=0.000),差异均有统计学意义。抑制SNHG1表达后,Ishikawa细胞的增殖和转移能力下降,细胞阻滞在G0/G1期,细胞中pPI3K和pAKT蛋白的表达减少。结论SNHG1在子宫内膜癌中表达上调,且其高表达水平与不良病理参数相关。干扰SNHG1可抑制子宫内膜癌的增殖和转移,SNHG1可能是通过调节PI3K/AKT信号通路参与子宫内膜癌的发生、发展。

Objective To investigate the expression and clinical significance of long non-coding RNA small nucleolar RNA host gene 1(lncRNA SNHG1)in endometrial carcinoma,and to explore the biological function of SNHG1 in endometrial carcinoma.Methods Real-time fluorescent quantitative PCR(qRT-PCR)was used to detect the expression level of SNHG1 in 54 cases of endometrial carcinoma and adjacent tissues,and the relationship between the expression level of SNHG1 in endometrial carcinoma and the clinicopathological parameters and its influence on the prognosis of endometrial carcinoma were analyzed.SNHG1 small-interfering RNA(siRNA)was transfected into the endometrial cancer cell line Ishikawa,which was divided into si-NC group and si-SNHG1 group.QRT-PCR was used to detect the effect of SNHG1 siRNA transfection.After interfering with the expression of SNHG1,the proliferation activity of the cells was detected by MTS assay,the cell cycle was detected by flow cytometry,the metastasis ability of the cells was detected by Transwell assay,and the activation of PI3K/AKT signaling pathway was detected by western blotting,the survival rate of population with SNHG1 high and low expression was compared.Results The expression of SNHG1 in endometrial carcinoma tissues was higher than that in adjacent tissues(t=5.236,P=0.000),the high SNHG1 expression was related to FLGO stage,depth of myometrial invasion and and lymph node metastasis(t=2.289~3.136,P=0.003~0.037),and the survival rate of endometrial carcinoma patients with high expression of SNHG1 was lower(χ^(2)=33.266,P=0.000),the differences were statistically significant,respectively.Inhibition of snhg1 expression decreased the proliferation and metastasis of Ishikawa cells,and the cell arrest was induced in the G0/G1 phase,and the expression of pPI3K and pAKT protein were decreased in the cells.Conclusion The expression of SNHG1 was up-regulated in endometrial carcinoma,and its high expression level was related to adverse pathological parameters.Interference with SNHG1 can inhibit the proliferation and metastasis of endometrial carcinoma.SNHG1 may be involved in the occurrence and development of endometrial carcinoma by regulating PI3K/Akt signal pathway.