SLC4A10表达水平对肝癌患者预后的影响

Effect of SLC4A10 expression on prognosis of patient with hepatocellular carcinoma

目的通过生物信息学(生信学)分析在肝细胞癌(HCC)中SLC4A10的表达对HCC患者预后的影响,初步验证并探讨其意义。方法通过TCGA数据库,分析SLC4A10在HCC组织与正常组织间的差异表达,并利用实时定量PCR进行验证。运用Kaplan-Meier(K-M)曲线分析SLC4A10的表达量对HCC患者预后的影响;通过单因素与多因素回归分析SLC4A10与HCC患者总生存期(CS)和无病生存期(DFS)的相关性;通过PPI数据库构建蛋白互作网络并进行功能富集分析,探索SLC4A10在HCC中的调控机制。结果通过生信学分析发现,SLC4A10在HCC组织中的表达显著低于正常组织;SLC4A10 mRNA表达降低与晚期临床病理参数显著相关,并降低HCC患者的CS和DFS;功能富集分析显示SLC4A10参与的重要的生物过程包括跨上皮转运、细胞体积稳态和钠离子跨膜导入。结论通过生信学分析提示SLC4A10在HCC中表达降低,其高表达利于HCC患者的预后,可能是HCC的潜在预后标志物或治疗靶点。

Objective To analyze the effect of SLC4A10 expression on the prognosis of patients with hepatocellular carcinoma(HCC)by bioinformatics,and to confirm its expression in HCC tissue and normal tissue.Methods The expression of SLC4A10 in HCC and in normal tissues was analyzed by TCGA database,and verified by real-time quantitative PCR.Kaplan-Meier(K-M)curve was applied to assess survival profile.Univariate and multivariate Cox regression analysis was applied to address the influence of independent factors on overall survival(OS)and disease free survival(DFS).PPI database was used to construct protein interaction network and function enrichment analysis was used to explore the regulatory mechanism of SLC4A10 in HCC.Results Significantly decreased transcriptional SLC4A10 expression was found in HCC samples.Decreased SLC4A10 mRNA expression was significantly associated with advanced pathological parameters and with shorter OS and DFS in TCGA cohorts.Functional annotations indicated that SLC4A10 was involved in the most significant hallmarks including transepithelial transport,cell volume homeostasis and sodium ion import across plasma membrane.Conclusions The decreased SLC4A10 expression is significantly correlated with aggressive progression and poor survival of HCC patients.These data suggest that SLC4A10 may act as a promising prognostic marker or therapeutic target in HCC.