全基因组选择信号揭示绵羊毛囊发育及脱毛性状相关的候选基因

Genome-wide Selection Signals Reveal Candidate Genes Associated with the Sheep of Hair Follicle Development and Depilation Traits

利用全基因组选择信号研究不同羊毛类型绵羊的群体结构及遗传分化程度,以挖掘与毛囊发育及脱毛性状相关的候选基因。本研究以3种羊毛类型的21个品种共290只绵羊群体为研究对象,利用Illumina Ovine SNP 50K芯片基因分型数据,基于群体分化指数Fst和核苷酸多样性比值θπRatio方法对无绒毛型、细毛型和中毛型绵羊进行选择信号检测。将Fst和θπRatio的top 5%作为阈值检测受到强烈选择的SNPs位点并进行注释。结果表明,SOX18、ALX4、FGF1和LRP4等与毛囊发育及脱毛性状相关的基因受到强烈选择。本研究通过Fst和θπRatio两种方法检测出与毛囊发育周期、羊毛形成以及毛囊和皮脂腺部分细胞相关的重要基因,这将为进一步研究绵羊重要经济性状形成的机制提供有意义的参考。

In order to discover candidate genes related to hair follicle development and depilation traits,genome-wide selection signals were used to study the population structure and genetic differentiation of sheep with different wool types.In current study,the population of 290 sheep from 21 breeds with three types of wool was selected as research object,using Illumina Ovine SNP 50K microarrays genotyping data,based on Fst andθπRatio methods,the selection signal detection of hair sheep,fine wool sheep and medium wool sheep were detected.Apply the top 5%of Fst andθπRatio as the threshold to detect and annotate the strongly selected SNPs.The results showed that genes related to hair follicle development and hair loss traits such as SOX18,ALX4,FGF1 and LRP4 were strongly selected.In this study,two methods of Fst andθπRatio were adopted to detect important genes related to hair follicle development cycle,hair formation,and some cells of hair follicles and sebaceous glands.This will provide a meaningful reference for further research on the mechanism of important economic traits in sheep.