7株鸡滑液囊支原体四川分离株全基因组分析

Whole Gene Analysis of 7 Mycoplasma synoviae Strains from Chicken

旨在了解滑液囊支原体(Mycoplasma synoviae,MS)遗传多样性,本研究对7株MS四川分离株进行全基因组测序及生物信息学分析。采用Illumina HiSeq平台和PE文库结合方式全基因组测序,运用各数据库注释,用MEGAX软件将7株MS与NCBI收录的8株MS全基因比较。结果显示,分离自同一鸡场的4株MS分离株MS251、MS254、MS221、MS231的基因数、种类、毒力因子数相同或差异小,而分离自3个不同鸡场的MS分离株MS12H、MS1G、MSK1的基因数、毒力因子数存在较大差异;将该7株MS四川分离株与NCBI公布的8株MS分离株比较圈图,发现四川分离株与巴西MS53、美国WVU-1853、澳大利亚86079/7 NS,以及疫苗株MS-H差异较大,而与河南HN01最相近。不同场间和同场间四川分离株都存在遗传多样性。本研究为研究MS分离株的致病机制、开发MS亚单位疫苗和建立特异性检测方法提供了理论依据。

In order to understand the genetic diversity of Mycoplasma synoviae(MS),seven MS strains isolated from Sichuan were sequenced and analyzed by bioinformatics.Illumina HiSeq platform and PE library were combined for whole genome sequencing,and the whole genome were annotated by several database.The complete genomes of 7 MS isolates were compared with those of 8 MS strains included in NCBI by MEGAX software.The results showed that the gene numbers,species and virulence factors of 4 MS strains MS251,MS254,MS221,MS231 isolated from the same chicken farm were the same or had little difference,while the gene number and virulence factor numbers of MS isolates MS12H,MS1G and MSK1 isolated from other three different chicken farms were quite different.Comparing the 7 MS Sichuan isolates with the 8 MS strains published by NCBI,it was found that the Sichuan MS isolates were significantly different from Brazilian MS53,American WVU-1853,Australian 86079/7 NS and vaccine strain MS-H,however,most similar to Henan HN01,China.There was genetic diversity among MS isolates from different farms and within the same farm.This study provided a necessary theoretical basis for further study of the pathogenic mechanism of MS isolates,the development of MS subunit vaccine and the establishment of specific detection technology.