基于癌基因组图谱数据挖掘分析CC亚族趋化因子配体20在食管癌中的表达及临床意义

Expression and clinical significance of CC motif chemokine ligand 20 in esophageal carcinoma based on the Cancer Genome Atlas data mining

目的探讨CC亚族趋化因子配体20(CCL20)在食管癌中的表达及对病程进展和预后的影响。方法利用癌基因组图谱(TCGA)、UALCAN、GEO等相关数据库分析CCL20基因在食管癌组织与癌旁组织中的表达差异,通过Kaplan-Meier模型探讨CCL20表达水平与预后的关联性;分析CCL20与肥胖、甲基化等之间的关系;最后通过mRNA、蛋白水平,分析CCL20在食管癌组织和癌旁组织的表达差异。结果CCL20在食管癌尤其是腺癌类型中高表达,高表达CCL20患者生存率明显降低;CCL20的表达量与食管癌患者肥胖程度成正比、与其甲基化程度成反比;食管癌组织中CCL20的表达量显著增加。结论CCL20在食管癌组织中高表达,且CCL20高表达与患者的生存预后成显著负相关。

Objective To explore the expression and significance of CC mokif chemokine ligand 20(CCL20)gene in esophageal carcinoma and its influence on the progression and prognosis of the disease.Methods The expression data of esophageal cancer were obtained from the Cancer Genome Atlas(TCGA)database,and were collected from cBioPortal(www.cbioportal.org).The survival curve was analyzed by Kaplan-Meier method.Through the website(http://ualcan.path.uab.edu)the objective was to analyze the expression of CCL20 gene in esophageal cancer tissues,the relationship between CCL20 and obesity and the methylation level of CCL20 in esophageal cancer.cBioPortal was used to analyze the copy number and mRNA expression of CCL20 gene.Finally,the expression differences of CCL20 in esophageal cancer tissues and adjacent tissues were analyzed by mRNA and protein levels.Results CCL20 was highly expressed in esophageal cancer,especially adenocarcinoma.The survival rate of patients with high expression of CCL20 was reduced.The expression of CCL20 was directly proportional to the degree of obesity in patients with esophageal cancer and inversely proportional to the degree of methylation.The expression of CCL20 in esophageal cancer tissues increased significantly.Conclusion CCL20 gene expression level is associated with poor prognosis of esophageal cancer patients.