槐杞黄颗粒对肝癌患者的疗效观察及MMPs/TIMPs水平的影响

Observation on the therapeutic efficacy of Huaiqihuang granules on patients with liver cancer and its impacts on MMPs/TIMPs level

目的探讨槐杞黄颗粒治疗肝癌的临床疗效及对MMPs/TIMPs的影响。方法选取浙江省嘉兴市第一医院2018年11月至2020年11月肝癌患者90例,随机分为肝癌组、槐杞黄低剂量组、槐杞黄高剂量组,每组各30例,另选取15名健康者设为对照组。全自动生化仪检测研究对象ALT、AST、AFP水平,ELISA法检测血清MMP-2、MMP-9及TIMP-2和TIMP-1含量,观察各组症状改善、生活质量评分情况。结果肝癌组、槐杞黄低剂量组、高剂量组ALT、AST、AFP水平逐渐下降,但高于对照组,差异有统计学意义(P<0.001)。肝癌组、槐杞黄低、高剂量组血清MMP-2、MMP-9逐渐降低(P<0.05),但高于对照组(P<0.05),血清TIMP-2和TIMP-1含量逐渐升高(P<0.05),但低于对照组(P<0.05)。高剂量组临床症状改善率为80.00%,明显高于低剂量组和肝癌组(P<0.05)。槐杞黄高剂量组KPS评分较低剂量组和肝癌组升高,差异有统计学意义(P<0.05)。结论槐杞黄颗粒治疗肝癌能够有效改善肝功能,提高临床疗效,可能通过降低MMPs及TIMPs水平相关,值得临床广泛应用。

Objective To investigate the clinical efficacy of Huaiqihuang granules on liver cancer and its impacts on matrix metalloproteinases(MMPs)/tissue inhibitors of metalloproteinases(TIMPs).Methods A total of 90 patients with liver cancer admitted to the First Hospital of Jiaxing in Zhejiang Province from November 2018 to November 2020 were selected.They were randomly divided into the liver cancer group(n=30),the low-dose Huaiqihuang group(n=30)and the high-dose Huaiqihuang group(n=30),and another 15 healthy people were selected and set as the control group(n=15).The levels of alanine transaminase(ALT),aspartate transaminase(AST)and alpha-fetoprotein(AFP)of the subjects were detected by fully automatic biochemical analyzer,and the contents of MMP-2,MMP-9,TIMP-2 and TIMP-1 in serum were detected by the enzyme linked immunosorbent assay(ELISA)method.The improvement of symptoms and KPS score of quality of life in each group were observed.Results The levels of ALT,AST and AFP in the liver cancer group,the low-dose Huaiqihuang group and the high-dose Huaiqihuang group decreased gradually,which were relatively lower than those in the control group,with statistically significant differences(P<0.001).Serum MMP-2 and MMP-9 in the liver cancer group,the low-dose Huaiqihuang group and the high-dose Huaiqihuang group decreased gradually(P<0.05),but increased compared with the control group(P<0.05).Contents of the serum TIMP-2 and TIMP-1 increased gradually(P<0.05),but decreased compared with the control group(P<0.05).The improvement rate of clinical symptoms in the high-dose group was 80.00%,which was significantly higher than that in the low-dose group and the liver cancer group(P<0.05).The KPS score of the high-dose Huaiqihuang group was higher than those of the low-dose Huaiqihuang group and the liver cancer group,with statistically significant difference(P<0.05).Conclusion Huaiqihuang granules can effectively improve liver function and clinical efficacy in the treatment of liver cancer,which may be related to decreasing the levels of MMPs and TIMPs.Therefore,it is worthy of wide clinical application.