甲氧西林耐药表皮葡萄球菌的多重耐药表型分析 及抗菌中药筛选

Multi-drug Resistance Phenotype Analysis of Methicillin Resistant Staphylococcus Epidermidis and Screening of Antibacterial Chinese Medicine

目的考察甲氧西林耐药表皮葡萄球菌(MRSE)多重耐药表型,同时筛选对其有抗菌活性的中药。方法通过实验室体外强诱导方式获得MRSE,测定其对临床常用抗菌药物敏感性,确定其具有多重耐药表型,同时对53种中药进行体外抗菌活性的研究。结果表皮葡萄球菌对甲氧西林的MIC为2μg/ml,处于敏感水平,在甲氧西林诱导下表现为对苯唑西林、青霉素G、氨苄西林、四环素呈现多重耐药性;中药对MRSE抗菌结果表明,金银花、连翘对MRSE的最小抑菌浓度(MIC)均为2.5 mg/ml,败酱草、黄芩、野菊花、一点红对MRSE的MIC为5 mg/ml,大黄、蒲公英、爵床及六月雪对MRSE的MIC均为10 mg/ml。结论MRSE对苯唑西林、青霉素G、氨苄西林和四环素产生交叉耐药,表现为多重耐药表型,败酱草、黄芩、金银花、连翘、大黄、野菊花、蒲公英、一点红、爵床以及六月雪对MRSE具有抗菌活性。

Objective To investigate the multidrug resistant phenotype of methicillin resistant Staphylococcus epidermidis(MRSE),and to screen the antibacterial activity of traditional Chinese medicine(TCM)against MRSE.Methods Mutants were selected by serial passage in TSB medium containing successively increasing concentration of methicillin.53 kinds of Traditional Chinese medicines were selected to test antibacterial activity.Results The MIC of Staphylococcus epidermidis to methicillin was 2μg/ml,which was at a sensitive level.Under the induction of methicillin,Staphylococcus epidermidis showed multiple drug resistance to oxacillin,penicillin G,ampicillin and tetracycline.The antibacterial results of traditional Chinese medicine on MRSE showed that the minimum inhibitory concentration(MIC)of honeysuckle and forsythia forsythia on MRSE was 2.5 mg/ml.The MIC of Patrinia,Scutellaria baicalensis,Chrysanthemum indicum and A little red on MRSE was 5 mg/ml.The MIC of rhubarb,dandelion,jazz bed and snow in June on MRSE was 10 mg/ml.Conclusion MRSE has cross-resistance to oxacillin,penicillin G,ampicillin and tetracycline,showing multi-drug resistant phenotype.Patrinia,Scutellaria baicalensis,honeysuckle,Forsythia suspensa,Rhubarb,Chrysanthemum indicum,dandelion,A little red,jazz bed and June snow have antibacterial activity against MRSE.