甲状腺细针穿刺活检BRAF基因V600E检测对于中央区淋巴结转移的预测价值

目的研究甲状腺乳头状癌BRAF基因V600E突变是否与中央区淋巴结转移存在相关性。方法收集2020年1月至2021年1月在焦作市人民医院经术前超声引导下甲状腺细针穿刺活检(FNAB)标本,应用实时荧光定量聚合酶链式反应(PCR)方法检测BRAF基因V600E突变状态,术中行中央区淋巴结清扫术,术后病理确诊甲状腺乳头状癌患者,统计其BRAF基因V600E突变状态和中央区淋巴结转移的数目,分析其是否具有相关性。结果甲状腺细针穿刺活检BRAF基因V600E突变与中央区淋巴结转移不具相关性(P=0.138)。多因素Logistic回归分析显示:BRAF基因V600E突变状态与颈部中央区淋巴结转移不存在相关性(P=0.136,OR=0.387)。结论甲状腺细针穿刺活检BRAF基因V600E检测对中央区淋巴结转移不具有预测价值。

Objective To study whether there is a correlation between BRAF gene V600 E mutation and central lymph node metastasis in thyroid papillary carcinoma.Me thods From January 2020 to January 2021,thyroid fine needle aspiration biopsy(FNAB)specimens guided by preoperative ultrasound in Jiaozuo People’s Hospital were collected.The mutation status of BRAF gene V600 E was detected by real-time fluorescence quantitative polymerase chain reaction(PCR).Central lymph node dissection was performed during operation.Patients with thyroid papillary carcinoma were confirmed by pathology after operation,The status of BRAF gene V600 E mutation and the number of central lymph node metastasis were counted to analyze whether there was correlation.Re s ults There was no correlation between BRAF gene V600 E mutation and central lymph node metastasis in thyroid fine needle aspiration biopsy(P=0.138).Multivariate logistic regression analysis showed that there was no correlation between BRAF gene V600 E mutation and cervical central lymph node metastasis(P=0.136,OR=0.387).Conclus ion The detection of BRAF gene V600 E by fine needle aspiration biopsy of thyroid gland has no value in the prediction of central lymph node metastasis.