P-选择素mRNA及蛋白在良、恶性葡萄胎鉴别诊断中的临床意义

Clinical Significance of P-selectin mRNA and Protein in the Differential Diagnosis of Benign and Malignant Hydatidform Mole

目的探讨分析P-选择素(P-selectin)在良、恶性葡萄胎组织中的表达特点及临床意义。方法选取50例良性葡萄胎和22例恶性葡萄胎组织,采用实时荧光定量PCR(qRT-PCR)技术检测P-选择素mRNA表达水平。采用免疫组化SP法和蛋白印迹(Western Blot)技术综合检测P-选择素蛋白表达水平。结果与良性葡萄胎组织相比,恶性葡萄胎组织中P-选择素mRNA、蛋白相对表达量和蛋白阳性表达率显著增高(P<0.05)。结论P-选择素在基因、蛋白水平上均参与了葡萄胎恶性的发展,有望成为评价良、恶性葡萄胎的诊断指标,为临床靶向治疗提供新思路。

Objective To investigate the expression characteristics and clinical significance of P-selectin in benign and malignant hydatidiform mole tissues.Methods 50 cases of benign moles and 22 cases of malignant moles were selected.qRT-PCR technology is used to detect the expression level of P-selectin mRNA.Immunohistochemistry SP method and Western Blot technology comprehensively detected the expression level of P-Selectin protein.Results Compared with benign hydatidiform mole tissue,the relative expression of mRNA and protein and protein positive expression rate of P-selectin in malignant hydatidiform mole tissue were significantly higher(P<0.05).Conclusion P-selectin participates in the development of malignant hydatidiform mole at the gene and protein level,and is expected to become a diagnostic indicator for evaluating benign and malignant hydatidiform moles,providing new ideas for clinical targeted therapy.