MiR-4262 regulates differentiation and osteogenesis of humanperiodontal stem cells by targeting suppressor of cytokinesignaling 4

Periodontitis,as a chronic inflammatory disease,remains unsolved,and the pathogenesis of this disease has not been fully elucidated.In this study,the effect of miR-4262 was investigated in tumor necrosis factor-α(TNF-α)induced human periodontal stem cells(hPDLSCs)for the first time.The gene expression involved in this study was determined using polymerase chain reaction(PCR),the expressions of relevant proteins were determined by western blot analysis,and the levels of IL-1β,IL-6,and MCP-1 were estimated by enzyme-linked immunosorbent assay(ELISA)assay.The luciferase reporter assay was performed for verification of the target gene,alkaline phosphatase(ALP)activity detection was used for differentiation capacity,and alizarin red staining assay was used for mineralization capacity.The inhibition of miRNA-4262,which resulted in the upregulation of suppressor of cytokine signaling 4(SOCS4),showed protective effects,including anti-inflammation and promotional effects on osteogenesis as well as differentiation in TNF-αinduced hPDLSCs.These results provided insights into the roles of miRNAs in regulating the inflammatory response,differentiation,and osteogenesis in hPDLSCs,which may promote the understanding of the mechanisms of periodontitis and find out a better therapeutic application.