基于正交实验法优化从栀子黄色素中制备藏红花酸的工艺

Optimization of preparation process of crocetin from gardenia yellow pigment by orthogonal experiment

基于正交实验法,优化从栀子黄色素中提取制备藏红花酸的碱水解工艺,以期可以简单高效地获得高纯度藏红花酸。建立高效液相色谱法(HPLC)测定藏红花酸含量,以藏红花酸的含量和得率为考察指标,采用正交实验法考察碱水解工艺中的料液比、NaOH浓度、水解温度和水解时间对产品中藏红花酸含量和得率的影响。确定栀子黄色素碱水解的最佳条件:料液比1∶6 g/mL、NaOH浓度3 mol/L、水解温度55℃、水解时间60 min。在该条件下制备获得的藏红花酸得率可达15.33%±1.25%;含量可达到97.24%±0.78%。优化后方法步骤简单易行,绿色无污染,一步制得高纯度藏红花酸,适用于工业化生产。

To simply and efficiently prepare high-purity crocetin from gardenia yellow pigment,the alkaline hydrolysis process was optimized using an orthogonal experimental design.High-performance liquid chromatography(HPLC)was established for the determination of crocetin in the samples.The evaluation index was based on the purity and yield of crocetin.The solid-liquid ratio,NaOH concentration,hydrolysis temperature,and hydrolysis time were investigated.The optimal conditions for alkaline hydrolysis of gardenia yellow pigment were obtained as follows:solid-liquid ratio 1∶6 g/mL,NaOH concentration 3 mol/L,hydrolysis temperature 55°C,and hydrolysis time 60 min.Under this condition,the crocetin yield was 15.33%±1.25%and a content of 97.24%±0.78%.Finally,high-purity crocetin was prepared from the gardenia yellow pigment in a single step.This method is simple,environmentally friendly,and suitable for industrial application.