正交实验优化老鹳草中6种成分超声提取工艺

Orthogonal Experiment for Optimization of Ultrasonic Extraction Process of Six Components from Geranium wilfordii Maxim

目的采用L_(9)(3^(4))正交实验优化老鹳草中老鹳草素、没食子酸、柯里拉京、金丝桃苷、鞣花酸、原儿茶酸6种成分的超声提取方法。方法在单因素实验基础上,选取甲醇体积分数、超声提取时间、料液比3个主要因素进行L_(9)(3^(4))正交实验,研究各因素相互作用对老鹳草中6种成分提取率的影响。结果老鹳草中6种成分的超声法最佳提取工艺为:甲醇溶液体积分数:60%,料液比:1.0 g/10 mL,超声波提取时间:40 min。结论此方法操作简便,重复性高,准确度好,可用于老鹳草中老鹳草素、没食子酸、柯里拉京、金丝桃苷、鞣花酸、原儿茶酸6种成分的同时提取。

Objective T o optimize the ultrasonic extraction method for Geraniin,Gallic acid,Corilagin,Hyperoside,Ellagic acid,Protocatechuic acid from Geranium wilfordii Maxim by L_(9)(3^(4))orthogonal experiment.Methods On the basis of single factor experiments,using L_(9)(3^(4))orthogonal experiment,three main factors,methanol volume fraction,extraction time,solid-liquid ratio are chosen for three main component to study the effect of the interaction of various factors on the extraction rate of six components fromGeranium wilfordiiMaxim.Results The results show that the optimum extraction process are as follow:the methanol volume fraction was 60%,the solid-liquid ratio was 1∶10(g∶ml),the extraction time was 40 min.Conclusion This method is simple,repeatability and accuracy.It can be to extract Geraniin,Gallic acid,Corilagin,Hyperoside,Ellagic acid,Protocatechuic acidfrom Geranium wilfordii Maxim.