上皮性卵巢癌组织中EGFR、EGFR mRNA、LRP和LRP mRNA表达水平及其临床意义

Expression Levels and Clinical Significance of EGFR,EGFR mRNA,LRP and LRP mRNA in Epithelial Ovarian Cancer

目的探讨上皮性卵巢癌卵巢组织中表皮生长因子受体(EGFR)、卵巢组织中表皮生长因子受体信使RNA(EGFR mRNA)、肺耐药蛋白(LRP)和肺耐药蛋白信使RNA(LRP mRNA)表达水平及临床意义。方法选取经过手术治疗且确诊为上皮性卵巢癌的98例患者进行研究。另选择取同期体检为健康人群的30例女性作为对照组。通过用逆转录聚合酶链反应(RT-PCR)对EGFR、EGFR mRNA、LRP和LRP mRNA表达水平的表达进行检测。结果研究组的EGFR、LRP阳性表达率明显高于对照组,差异有统计学意义(P<0.05);研究组患者不同年龄、不同组织学分型、不同细胞学分级、是否有淋巴结转移的患者EGFR mRNA表达水平相比,差异无统计学意义(P>0.05);Ⅲ~Ⅳ期患者EGFR阳性表达率明显高于Ⅰ~Ⅱ期患者,差异有统计学意义(P<0.05)。研究组不同年龄、不同组织学分型、不同细胞学分级、不同病理分期、是否有淋巴结转移的患者LRP mRNA表达水平相比,差异无统计学意义(P>0.05)。在完成3个疗程后评定所有患者的化疗疗效,其中70例患者化疗有效,28例患者化疗无效。化疗有效组EGFR、LRP mRNA表达水平为(0.80±0.27,0.83±0.29),明显低于化疗无效组患者的(1.10±0.21,1.04±0.20),差异有统计学意义(P<0.05)。Pearson相关分析结果显示,EGFR与LRP mRNA表达呈正相关(γ=0.751,P<0.05)。结论卵巢上皮性癌组织中EGFR、EGFR mRNA、LRP和LRP mRNA表达水平均呈现不同程度的升高,且水平的变化情况能够有效对患者的化疗反应进行预测,能够有效帮助医生对临床个体化的治疗方案进行选择,有较为重要的临床意义。

Objective To investigate the expression and clinical significance of epidermal growth factor receptor(EGFR),epidermal growth factor receptor messenger RNA(EGFR mRNA),lung resistance protein(LRP) and lung resistance protein messenger RNA(LRP mRNA) in epithelial ovarian cancer.Methods 98 patients with epithelial ovarian cancer were selected. 30 healthy women were selected as the control group.The expression levels of EGFR,EGFR mRNA,LRP and LRP mRNA were detected by reverse transcription polymerase chain reaction(RT-PCR).Results The positive expression rates of EGFR and LRP in the study group were significantly higher than those in the control group(P<0.05);there was no significant difference in EGFR mRNA expression between the study group and the patients with different ages, different tissue types, different cytological grades and lymph node metastasis(P>0.05);the positive expression rate of EGFR in patients with stage Ⅲ~Ⅳ was significantly higher than that in patients with stage Ⅰ~Ⅱ(P<0.05).There was no significant difference in LRP mRNA expression between the study group and the patients with different age, different histological type, different cytological grade, different pathological stage and lymph node metastasis(P>0.05).After completing 3 courses of treatment, the chemotherapy efficacy of all patients was evaluated, of which 70 patients were effective and 28 patients were ineffective.The expression levels of EGFR and LRP mRNA in the effective chemotherapy group were(0.80±0.27,0.83±0.29),which were significantly lower than those in the ineffective chemotherapy group(1.10±0.21,1.04±0.20)(P<0.05).Pearson correlation analysis showed that there was a positive correlation between EGFR and LRP mRNA expression(γ=0.751,P<0.05).Conclusion The expression levels of EGFR,EGFR mRNA,LRP and LRP mRNA in epithelial ovarian cancer tissues were increased in varying degrees, and the changes of the levels can effectively predict the chemotherapy response of patients, and can effectively help doctors to choose the clinical individualized treatment scheme, which has more important clinical significance.