摘要
为探究连翘叶绿体基因组特征及该属物种的系统进化发育关系,利用高通量测序技术对连翘长花柱与短花柱植株叶绿体基因组进行测序和功能注释,利用PCR对高通量测序结果进行验证。结果显示:连翘长花柱与短花柱植株叶绿体基因组全长均为156 386bp,为典型的四段式结构。连翘叶绿体基因组注释到114个基因,包括80个蛋白编码基因,30个tRNA基因,4个rRNA基因。基因组56个简单重复序列(SSR)位点分析显示其SSR序列主要由A和T碱基组成。连翘叶绿体基因组编码区偏向使用A、T碱基,但整体密码子偏好性较弱,主要表现在Arg和Ser的差异。与已公布的32种植物(包括14种连翘属)叶绿体基因组序列构建系统发育进化树,结果显示连翘的叶绿体基因组与产自山西泽州的连翘高度同源。连翘异型花柱具有相同的叶绿体基因组序列,推测连翘的异型花柱是由同型花演化而来。
In order to explore the chloroplasts genomic characteristics of Forsythia suspensa and the phylogenetic development of this genus,high-throughput sequencing technology was used to sequence and functionally annotate the chloroplast genomes of long and short style type in F. suspensa. PCR was used to confirm the sequencing results. The results showed that the chloroplast genomes of the long and short styles type were 156 386 bp,which was a typical four-segment structure in F. suspensa. A total of 114 unique genes were annotated,including 80 protein-coding genes,30 tRNA,and four rRNA. Analysis of 56 simple sequence repeats(SSRs)showed that it mainly composes of A and T bases. In addition,the coding region of the chloroplast genome of F. suspensa prefers to use A and T bases,but the overall codon preference is weak,which is mainly reflected by the difference of Arg and Ser. Based on the chloroplast genome sequence of the heterostyly and 32 plants’ chloroplast genome sequences(including 14 species of Forsythia)published the phylogenetic relationship is analyzed. And the results showed that the chloroplast genome of heterostyly was highly homologous to F. suspensa in Zezhou,Shanxi. It is possible that heterostyly also evolved from the homostyly flowers in F. suspensa.
作者
宋芸
贾孟君
曹亚萍
李政
贺嘉欣
王勇飞
张鑫瑞
乔永刚
SONG Yun;JIA Mengjun;CAO Yaping;LI Zheng;HE Jiaxin;WANG Yongfei;ZHANG Xinrui;QIAO Yonggang(College of Life Sciences,ShanXi Agricultural University,Taigu,Shanxi 030801,China)
出处
《园艺学报》
CAS
CSCD
北大核心
2022年第1期187-199,共13页
Acta Horticulturae Sinica
基金
山西省基础研究计划面上项目(20210302123390)。