摘要
三萜类化合物是植物代谢产物中最具多样性的化合物之一,具有广泛的生理活性和重要的经济价值。环氧角鲨烯环化酶(oxidosqualene cyclases,OSCs)催化2,3-氧化鲨烯环化生成不同类型的甾醇和植物三萜化合物,对天然产物的结构多样性具有重要意义。然而,目前对于OSCs酶催化2,3-氧化鲨烯发生环化多样性的机理尚不清晰。文中总结了近几年OSCs酶的研究进展,主要包括以下几个方面:催化功能;基因与蛋白的分子进化关系;OSCs酶蛋白结构;分子模拟与分子计算。期望为三萜环化酶的蛋白质工程和代谢工程的研究奠定基础。
Triterpenoids are one of the most diverse compounds in plant metabolites,and they have a wide variety of physiological activities and are of important economic value.Oxidosqualene cyclases catalyze the cyclization of 2,3-oxidosqualene to generate different types of sterols and plant triterpenoids,which is of great significance to the structural diversity of natural products.However,the mechanism of the diversified cyclization of 2,3-oxidosqualene catalyzed by oxidosqualene cyclases remains unclear.This review summarized the research progress of oxidosqualene cyclases from the aspects of catalytic function,molecular evolutionary relationship between genes and proteins,protein structure,molecular simulation and molecular calculations,which may provide a reference for protein engineering and metabolic engineering of triterpene cyclase.
作者
陈翠玉
庞亚如
陈泉冰
李春
吕波
CHEN Cuiyu;PANG Yaru;CHEN Quanbing;LI Chun;LÜ Bo(Institute of Biochemical Engineering,School of Chemistry and Chemical Engineering,Beijing Institute of Technology,Beijing 102488,China;Department of Chemical Engineering,Tsinghua University,Beijing 100084,China)
出处
《生物工程学报》
CAS
CSCD
北大核心
2022年第2期443-459,共17页
Chinese Journal of Biotechnology
基金
国家重点研发计划(2018YFA0901800)
天津合成生物技术创新能力提升项目(TSBICIP-CYFH-002)
北京理工大学青年教师学术启动计划。
关键词
三萜化合物
环氧角鲨烯环化酶
分子进化
催化机理
分子模拟
triterpenoids
oxidosqualene cyclase
molecular evolution
catalytic mechanism
molecular simulation