A组轮状病毒与腹泻型大肠埃希菌混合感染致使婴幼儿肠道疾病的相关研究

Study on intestinal diseases in infants and young children caused by mixed infection of group A rotavirus and diarrhoeal Escherichia coli

目的探讨A组轮状病毒与腹泻型大肠埃希菌混合感染致使婴幼儿肠道疾病的临床关系。方法 100例腹泻型肠道疾病婴幼儿,均进行细菌培养,然后对培养所得大肠杆菌进行血清分型和β-半乳糖苷酶试验(ONPG试验),检测患儿A组轮状病毒和各血清型大肠埃希菌感染情况。根据实验结果将患儿分为对照组(单纯腹泻型大肠埃希菌感染,50例)及观察组(A组轮状病毒与腹泻型大肠埃希菌混合感染,50例)。比较两组肠道损害发生情况,ONPG试验培养结果[肠致病性大肠杆菌(EPEC)、产肠毒素大肠杆菌(ETEC)、侵袭性大肠杆菌(EIEC)、肠出血性大肠杆菌(EHEC)、肠聚集性大肠杆菌(EaggEC)阳性检出情况],分析患儿的年龄分布情况。结果观察组患儿的肠道损害发生率92.0%显著高于对照组的64.0%,差异有统计学意义(P<0.05)。观察组的ETEC、EPEC、EIEC、EHEC、EaggEC阳性率分别为32.0%、22.0%、18.0%、16.0%、12.0%,均显著高于对照组的14.0%、8.0%、4.0%、4.0%、0,差异具有统计学意义(P<0.05)。100例患儿中1~5月龄患儿17例(17.0%),6~12月龄患儿55例(55.0%),1~2岁患儿28例(28.0%);6~12月龄患儿占比最高,其次是1~2岁、1~5月龄患儿。结论 A组轮状病毒与腹泻型大肠埃希菌混合感染致使婴幼儿肠道疾病感染水平明显增高,同时轮状病毒对各型大肠埃希菌的生长繁殖有促进作用,因此,临床上应引起重视并做到早发现、早治疗,避免漏诊、误诊,早期控制病情,以减少对患儿的身心损害,值得临床推广。

Objective To discuss the clinical relationship of intestinal diseases in infants and young children caused by mixed infection of group A rotavirus and diarrhoeal Escherichia coli.Methods 100 infants and young children with diarrhea-type intestinal diseases were selected as the study subjects.The bacteria were cultured,and then the cultured Escherichia coli was serotyped and β-Galactosidase test(ONPG test) was used to detect the infection of group A rotavirus and various serotypes of Escherichia coli of the children.According to the experimental results,the children were divided into control group(50 cases of simple diarrhea Escherichia coli infection) and observation group(50 cases of mixed infection of group A rotavirus and diarrhoeal Escherichia coli).The incidence of intestinal damage,the culture results of ONPG test[positive detection of Enteropathic Escherichia coli(EPEC),enterotoxigenic Escherichia coli(ETEC),enteroinvasive Escherichia coli(EIEC),enterohemorrhagic Escherichia coli(EHEC) and enteroaggregative Escherichia coli(EaggEC)] were compared between the two groups,and the age distribution of children was analyzed.Results The incidence of intestinal damage of the observation group was 92.0%,which was significantly higher than 64.0% of the control group,and the difference was statistically significant(P<0.05).The positive rates of ETEC,EPEC,EIEC,EHEC and EaggEC of the observation group were 32.0%,22.0%,18.0%,16.0% and 12.0%,respectively,which were significantly higher than 14.0%,8.0%,4.0%,4.0% and 0 of the control group,respectively;and the differences were statistically significant(P<0.05).Among the 100 children,17 cases(17.0%) were 1-5 months old,55 cases(55.0%)were 6-12 months old and 28 cases(28.0%) were 1-2 years old.The proportion of children aged 6-12 months was the highest,followed by the proportions of children aged 1-2 years and 1-5 months.Conclusion The mixed infection of group A rotavirus and diarrhoeal Escherichia coli significantly increases the infection level of intestinal diseases in infants and young children.At the same time,rotavirus can promote the growth and reproduction of various serotypes of Escherichia coli.Therefore,attentions should be paid in clinical practice,so as to achieve early detection and treatment to avoid missed diagnosis,misdiagnosis and early control of the disease,in order to reduce the physical and mental damage to children.Thus,it is worthy of promotion in clinical practices.