甘蓝型油菜phytocyanin家族全基因组鉴定和表达分析

Genome-wide identification and expression analysis of phytocyanin family in Brassica napus

质体蓝素(phytocyanin,PC)是一类含铜电子转移蛋白,参与植物光合作用、生长发育以及对环境的适应等。为研究异源四倍体作物甘蓝型油菜的PC基因功能,对BnPC家族进行全基因组鉴定,共鉴定到173个成员。根据铜结合位点以及糖基化位点的差异将该基因家族的蛋白划分为4个亚家族:类早期结瘤素蛋白(BnENODL)、类花青苷蛋白(BnUCL)、类漆树蓝蛋白(BnSCL)和类质体蓝素蛋白(BnPLCL)。蛋白质序列特征分析结果表明,133个BnPC含有N端信号肽,77个BnPC具有糖基磷脂酰肌醇锚定信号,87个BnPC具有阿拉伯半乳聚糖结构域,104个BnPC具有N-糖基化位点。共线性分析结果表明,基因组复制和片段重复在该家族的扩张中起着重要作用。表达谱分析结果表明,部分BnPC基因的表达具有组织/器官特异性,95个BnPC基因至少对旱、盐、冷和高温胁迫中的一种产生响应。

Phytocyanin(PC)is a class of copper containing electron transfer proteins involving in plant photosynthesis, growth and environmental adaptation. To investigate the function of BnPC family in allotetraploid crop Brassica napus L., 173 members were identified from B. napus genome. They were divided into 4 subfamilies as early nodulin-like proteins, uclacyanin-like protein, stellacyanin-like protein, and plantacyanin-like protein. Structure analysis showed that among them, 133 BnPCs contain N-terminal secretion peptide, 77 contain glycosylphosphatidylinositol anchor signals which anchor PCs in cell membrane, 87 contain arabinogalactan protein-like regions, and 104 contain N-linked glycosylation sites. Whole genome duplication and segmental duplication played important roles in family expansion. Tissue/organ-specific pattern was found in their expression. 95 BnPCs were observed responding to at least one abiotic stress treatment including drought, cold, salt and heat.