miR-599在宫颈癌患者中的表达变化及其预后价值研究

Expression and prognostic value of miR-599 in patients with cervical cancer

目的探讨miR-599的表达对于宫颈癌患者临床病理及预后的价值。方法选取2018年1月至2021年1月本院收治的宫颈癌患者60例,收集患者癌组织标本及癌旁组织标本,以定量荧光检测法检测两者miR-599表达水平,使用CCK-8法检测细胞的增殖和侵袭能力,统计患者的不同miR-599水平表达与临床病理参数、预后生存率之间的关系。结果宫颈癌患者癌性组织内miR-599的表达明显高于癌旁正常组织,差异有统计学意义(P<0.05);miR-599的表达与宫颈癌FIGO分期、宫颈癌病理组织分化有关(P<0.05);在下调Hela细胞中的miR-599表达后,采用CCK-8法从第48小时开始,Hela细胞的增殖能力明显低于对照组(P<0.05),侵袭下室的细胞数明显少于对照组(P<0.05);miR 599高表达者3年期存活率低于低表达者(P<0.05)。结论宫颈癌患者癌性组织中miR-599的表达明显高于非癌组织,miR-599的表达与病理组织分化、细胞的增殖与侵袭能力、患者存活率密切相关,可作为诊断及治疗中的预测因子。

Objective To investigate the value of miR-599 expression in clinicopathology and prognosis of cervical cancer.Methods 60 pa-tients with cervical cancer admitted to our hospital from January 2018 to January 2021were selected.The samples of cancer tissues and adjacent tis-sues were collected.The expression levels of miR-599 were detected by quantitative fluorescence detection.The proliferation and invasion ability of cells were detected by CCK-8 method.The relationship between the expression of different miR-599 levels in patients with clinicopathological pa-rameters,prognosis and survival rate.Results The expression of miR-599 in cervical cancer tissues was significantly lower than that in adjacent nor-mal tissues(P<0.05),the expression of miR-599 was related to FIGO stage and pathological differentiation of cervical cancer(P<0.05);after down regulating the expression of miR-599 in Hela cells,CCK-8 method was used to detect the proliferation of Hela cells from the 48 h(P<0.05),while the number of cells invading the inferior chamber was significantly lower than that in the control group(P<0.05);the 3-year survival rate of high ex-pression of miR-599 was lower than that of low expression group(P<0.05).Conclusion The expression of miR-599 in cancerous tissues of cervi-cal cancer patients is significantly lower than that in non cancerous tissues.The expression of miR-599 is closely related to pathological differentia-tion,cell proliferation and invasion,and survival rate of patients,which can be used as a predictor in diagnosis and treatment.