尿中红细胞两种检测方法评价及应用

Evaluation and application of two methads for the determination of red blood cells in urine

目的评价干化学尿液分析仪UA-5800与尿液有形成分分析仪EH-2080C联合用于尿红细胞检测的准确性及符合程度, 为制定尿红细胞检测的过筛和复检标准提供参考。方法于2019年6月, 使用全自动尿液分析流水线EU-8000(由干化学尿液分析仪UA-5800及尿有形成分分析仪EH-2080C组成)分别对我院2 336例职业体检新鲜尿液标本进行尿隐血和尿红细胞检测, 分析两仪器检测结果的灵敏度(真阳性率)、特异度(真阴性率)及检测结果的阳性符合率(阳性率)、阴性符合率(阴性率)。结果干化学尿液分析仪UA-5800检测的尿隐血与尿液有形成分分析仪EH-2080C检测的尿红细胞灵敏度分别为98.33%、95.00%, 差异无统计学意义(χ^(2)=0.50、P>0.05);特异度分别为82.47%、97.45%, 差异有统计学意义(χ^(2)=337.05、P<0.01);两种方法对比, 两者差异有统计学意义(χ^(2)=339.05、P<0.01)。在干化学尿液分析仪UA-5800检测的尿隐血全部结果比较中, 尿液有形成分分析仪EH-2080C检测的尿红细胞阴性符合率最高(99.89%);而在尿隐血阳性结果比较中, 3+时尿液有形成分分析仪EH-2080C检测的尿红细胞阳性符合率最高(85.76%);当尿隐血结果分别为弱阳、1+、2+时, 尿液有形成分分析仪EH-2080C检测的阳性符合率偏低, 分别为8.97%、14.40%和36.48%, 但随阳性程度渐增而增高。结论本实验室尿红细胞筛选和复检标准, 对全自动尿液分析流水线筛选出的阳性标本进行金标准显微镜镜检, 可确保尿液红细胞分析结果的高准确和实现检测高效率。

Objective This study was aimed to evaluate the accuracy and conformity of the dry chemical urine analyzer UA-5800 and the urine formed element analyzer EH-2080Cused in the detection of urine red blood cells,and establish screening and retesting standards for urine red blood cell testing.Methods Automatic urine analysis line EU-8000(composed of dry chemical urine analyzer UA-5800 and urine formed element analyzer EH-2080C)was used to conduct hematuria and urine erythrocyte test in 2336 fresh urine samples from occupational medical examinations in our hospital,the sensitivity(true positive rate),specificity(true negative rate)and the positive coincidence rate(positive rate)and negative coincidence rate(negative rate)of the corresponding test results were analyzed tow instruments.Results The sensitivity of urinary occult blood detected by UA-5800 and urinary erythrocytes examined by EH-2080C were 98.33%and 95.00%,there was no statistical significant between the two results(χ^(2)=0.50、P>0.05);specificity was 82.47%and 97.45%respectively,which existed statistical significant(χ^(2)=337.05、P<0.01).Comparing the two methods,the statistical significant remained(χ^(2)=339.05、P<0.01).Among all results of urinary occult blood detected,the EH-2080C had the highest urinary erythrocyte negative coincidence rate(99.89%);while in the comparison of urinary occult blood positive grades at 3+,the highest positive coincidence rate of urinary erythrocyte in EH-2080C was 85.76%;when the urine occult blood levels were weakly positive,1+,2+,the positive coincidence rate of EH-2080C examination was low(8.97%,14.40%and 36.48%),which increased with elevated positive grade.Conclusion The positive specimens screened by automatic urine analysis line were examined by gold standard microscope combining with with the criteria of urine red blood cell screening and retesting in our laboratory,which can ensure high accuracy and high efficiency of urine erythrocyte analysis.