荧光偏振调制显微成像技术研究进展

Research Progress of Fluorescence Polarization Modulation Microscopy Imaging Technology

传统荧光显微成像技术以荧光强度为成像对比度,在获取生物分子位置和浓度信息中具有重要作用,为分子层面的生物医学研究提供了成像工具。而偏振作为荧光的另一重要特性,可以在另一维度提供分子的方向和结构信息,已被广泛应用于荧光偏振显微成像技术中。除此之外,荧光偏振调制也可以通过增强荧光图像稀疏性进而增强图像对比度来获取超分辨尺度的生物分子的位置和方向信息。从物理基础、技术原理、基本实现装置和生物应用等方面,总结了基于荧光偏振特性的不同荧光偏振调制成像技术在分子方向结构成像、超分辨显微成像以及二者的结合方面的发展概况,并对该技术未来的发展方向进行了展望。

The traditional fluorescence microscopy imaging technique takes fluorescence intensity as imaging contrast, which plays an important role in obtaining the position and concentration information of biomolecules and provides an imaging tool for biomedical research at the molecular level. As another important characteristic of fluorescence, polarization can provide the orientation and structural information of biomolecules in another dimension and has been widely used in the fluorescence polarization microscopy imaging technique. In addition, fluorescence polarization modulation can also be used to obtain the position and orientation information of biomolecules at the super-resolution scale by enhancing the sparsity of fluorescence images and thus enhancing the image contrast. In this paper, as for the different fluorescence polarization modulation imaging techniques based on fluorescence polarization characteristics, their developments in the molecular orientation structure imaging, super-resolution microscopy imaging and the combination of the two aspects are summarized from the physical bases, technical principles, basic implementation devices, and biological applications. Moreover, the future of these techniques is prospected.