霸王鞭乙酸乙酯部分化学成分研究

Study on chemical constituents from ethyl acetate extract of Euphorbia royleana Boiss

为了研究霸王鞭(Euphorbia royleana Boiss.)的化学成分及其卤虫致死活性,采用正相硅胶柱色谱、Sephadex LH-20凝胶柱色谱及半制备型高效液相色谱等方法从霸王鞭甲醇提取物的乙酸乙酯萃取部位中分离得到15个化合物。通过现代波谱学技术鉴定它们的结构分别为5(6)-guten-3α-ol(1)、蒲公英赛醇(2)、异蒲公英赛醇(3)、熊果酸(4)、齐墩果酸(5)、1-羟基-3,7,8-三甲氧基黄酮(6)、甲基獐牙菜素(7)、槲皮素(8)、胡萝卜苷(9)、β-谷甾醇(10)、豆甾醇(11)、香草醛(12)、十二烷醇(13)、植物醇(14)、1-[(12 E,16 E)-12,16-二十碳二烯酰基]-2-[(E,E)-7,11-十八碳二烯酰基]-3-硬脂酰基甘油(15)。化合物1、3、4、5、6、7、12、13、14和15均首次从该植物中分离得到。对所得到的化合物进行了卤虫致死活性研究,发现化合物4表现出较强卤虫致死活性,其LD_(50)为7.687μM。

To study the chemical composition of Euphorbia royleana Boiss.and the brine shrimp lethal activity, 15 compounds were isolated from the ethyl acetate extract of this plant by normal phase silica gel, sephadex LH-20 and semi-preparative high-performance liquid chromatography(HPLC).Their structures were identified by modern spectroscopic techniques as 5(6)-gluten-3α-ol(1),taraxerol(2),epitaraxerol(3),ursolic acid(4),oleanolic acid(5),1-hydroxy-3,7,8-trimethoxyxanthone(6),methylswertianin(7),quercetin(8),daucosterol(9),β-sitosterol(10),stigmasterol(11),vanillin(12),1-dodecanol(13),trans-phytol(14),1-[(12E,16E)-12,16-eicosadienoyl]-2-[(E,E)-7,11-octadecadienoyl]-3-stearoylglycerol(15).Compounds 1,3,4,5,6,7,12,13,14 and 15 were isolated from this plant for the first time.Through the brine shrimp lethal activity test, compound 4 showed strong brine shrimp lethal activity, LD_(50) was 7.687 μM.