心脏类器官的研究进展及其临床应用

Advances in research and application of cardiac organoids

心血管疾病是对人类健康的严重威胁,也是全球死亡的主要原因。传统的二维(two dimensional,2D)细胞模型及动物模型具有各自的局限性,不能完全适用于心血管疾病的发病机制及治疗研究。类器官是一种在体外三维(threedimensional,3D)培养并模拟体内器官的细胞结构,可以更准确地保留体内细胞的生物学特性和功能,为心血管疾病的研究开辟了新的方向与思路。目前众多研究报道了心脏类器官的构建方法及其在药物实验、疾病模型、再生医学与治疗等方面的应用。文章概述了心脏类器官的构建方法及其在临床上的应用,同时也提出了其发展前景和局限性。

Cardiovascular disease is a serious threat to human health,and it is also the main cause of death in the world.The traditional two dimensional(2D)cell model and animal model have their own limitations,which cannot be fully applied to the mechanism and treatment of cardiovascular disease.Organoid is a kind of three dimensional(3D)culture in vitro and simulates the cell structure of organ in vivo,which can more accurately retain the biological characteristics and functions of cells in vivo.It is a new direction and idea for the research of cardiovascular disease.Now,many studies have reported the construction methods of cardiac organs and their applications in drug experiments,disease models,regenerative medicine in treatment and so on.In this review,it was summarized the construction methods of cardiac organs and their clinical applications,and also put forward their development prospects and limitations.