基质固相分散和HPLC-IT-TOF/MS联用研究漆树木材中多酚成分

Study on Polyphenols in Lacquer Tree by Matrix Solid-phase Dispersion and HPLC-IT-TOF/MS

采用基质固相分散(MSPD)技术进行漆树木中多酚化合物的提取,根据单因素试验得到优化工艺条件为:ZSM-22为吸附基质,吸附基质与样品的质量比为2∶1,洗脱溶剂为体积分数70%甲醇,洗脱溶剂用量8 mL,研磨时间2 min。在此条件下,漆树木多酚提取得率为(466±2.05)mg/g(以没食子酸计)。采用高效液相色谱-离子阱-飞行时间质谱(HPLC-IT/TOF-MS)联用技术,建立高效、准确鉴定漆树木中多酚化学成分的方法,根据质谱图和裂解规律信息,并结合文献,共鉴定10个化合物,包括3个没食子酸(GAC)类化合物和7个黄酮类化合物,分别是没食子酸、没食子酸甲酯、没食子酸乙酯、黄颜木素、花旗松素、3,4′,7-三羟基黄烷酮、异鼠李素、槲皮苷、漆黄素和硫黄菊素。利用基质固相分散和HPLC-IT/TOF-MS技术有利于漆树木中化合物的发现和鉴别,对漆树提取物的质量评价和后续分析具有重要的意义。

Matrix solid-phase dispersion was proposed to extract polyphenols in Toxicodendron vernicifluum(Stokes)F.A.Barkl.(Shaanxi lacquer tree).The MSPD conditions to extract polyphenols established by the single factor test were ZSM-22 as the adsorbent,adsorbent/sample ratio of 2∶1(g∶g),grinding for 2 min,and use of 8 mL of 70%methanol as the elution solvent.Under these conditions,the polyphenols content of MSPD extraction was(466±2.05)mg/g(calculated by gallic acid).An efficient and accurate method for identification of polyphenols in Shaanxi lacquer tree was established by HPLC-IT-TOF/MS.According to mass spectrometry,fragmentation pathway combined with published reports,total of 10 components were identified,including 3 gallic acid derivatives and 7 flavonoids as follows:gallic acid,methyl gallate,ethyl gallate,fustin,taxifolin,garbanzol,isorhamnetin,quercitrin,fisetin,sulfuretin.It was beneficial to the discovery and identification of compounds in Shaanxi lacquer tree by matrix solid-phase dispersion coupled with HPLC-IT-TOF/MS,and it could be significance for the analysis and quantification of extracts from Shaanxi lacquer tree samples.