植物乳杆菌HCS03-001安全性评价及其益生特性分析

Safety Evaluation and Probiotic Characteristics Analysis of Lactobacillus plantarum HCS03-001

为了对植物乳杆菌HCS03-001(CGMCC No.16258)进行安全性评价以及益生特性的研究,通过溶血实验、耐药实验及急性经口毒性实验评价其安全性,通过对酸环境及胆盐环境的耐受性实验、抑菌功能特性实验及通便功效试验评价该菌株的益生特性。结果表明,植物乳杆菌HCS03-001在哥伦比亚血琼脂培养基上无透明圈形成,即无溶血性;植物乳杆菌HCS03-001对青霉素(PEN)、氨苄西林(AM)、亚胺培南(IP)、美罗培南(MP)、利奈唑胺(LZ)、达托霉素(DPC)敏感,对红霉素(EM)中敏,对万古霉素(VA)耐药;小鼠经口急性毒理无毒,确定该菌株为食用安全菌。该菌株在pH3.0和pH2.0环境中培养3 h存活率分别为94.29%和92.33%,在1.5 g/L胆盐浓度下培养3 h,存活率仍能达到88.82%;该菌对肠道致病菌及口腔致病菌(牙龈卟啉单胞菌和变异链球菌)均有抑制作用;对盐酸洛哌丁胺的便秘模型有较好的促排便效果。植物乳杆菌(Lactobacillus plantarum)HCS03-001具有良好的安全性及益生特性。

In order to evaluate the safety and probiotic properties of Lactobacillus plantarum HCS03-001(CGMCC No.16258),the safety of Lactobacillus plantarum HCS03-001 was evaluated by hemolysis test,drug resistance test and acute transoral toxicity test.The probiotic properties of the strain were evaluated by acid and bile salt tolerance test,bacteriostatic functional properties test and laxative efficacy test.The results showed that Lactobacillus plantarum HCS03-001 did not form transparent circle on the Colombian blood finger culture medium,that was,there was no hemolysis.Lactobacillus plantarum HCS03-001 was sensitive to penicillin(PEN),ampicillin(AM),imipenem(IP),meropenem(MP),linezolid(LZ),and datoramycin(DPC),and neutral to erythromycin(EM) and resistant to vancomycin(VA).The acute oral toxicology of mice was non-toxic,and the strain was confirmed to be safe to eat.The survival rate of the strain was 94.29%and 92.33% at pH3.0 and pH2.0 for 3 h,respectively.The survival rate could still reach 88.82% at 1.5 g/L bile salt for 3 h.It had inhibitory effect on intestinal and oral pathogens(Porphyromonas gingivalis and Streptococcus mutans).It had a good effect on promoting defecation in constipation model of loperamide hydrochloride.Lactobacillus plantarum HCS03-001 has good safety and probiotics.