摘要
本研究以魔芋葡甘聚糖(konjac glucom annan,KGM)为壁材,原花青素(proanthocyanidins,PC)为芯材,利用KGM在乙醇体系下吸水微溶胀的特性制备载PC的KGM微粒,为PC结肠靶向递送提供新的技术思路。实验利用KGM吸附溶于乙醇水溶液中的PC这一方法来制备KGM/PC微粒,采用单因素实验及正交试验优化KGM/PC微粒的制备工艺,通过粒径分析、扫描电镜、红外光谱等对微粒的性质进行表征,并考察了该微粒体外释放性能。结果表明,KGM能成功包封PC,且PC的负载率受KGM及PC添加量的显著影响;正交优化确定KGM负载PC的最佳工艺条件为:在10%(v:v)乙醇溶液中添加16%(w:v)的KGM(粒径为50~90μm)及11%(w:v)PC时,微粒对PC的负载率为(14.75%±0.27%);同时,经过胃和小肠各消化4 h后,KGM/PC微粒中的PC有79.47%未被释放,可到达结肠部位。综上,该包封微粒在结肠靶向递送方面具有较大的潜力,可为工业生产提供一种简单、有效、安全的包封技术。
In this study,konjac glucomannan was used as wall material and proanthocyanidins as core material.The microswelling properties of KGM in the ethanol system were used to prepare PC-loaded KGM microparticles,which provided a new technical idea for colon-targeted delivery of PC.KGM/PC microparticles were prepared by KGM adsorption of PC dissolved in ethanol aqueous solution.Single factor experiment and orthogonal experiment were used to optimize the preparation process of KGM/PC microparticles,and the properties of the microparticles were characterized by particle size analysis,scanning electron microscopy,infrared spectroscopy,etc.The release performance of the microparticles in vitro was investigated.The results showed that KGM could successfully encapsulate PC and the loading rate of PC was significantly affected by KGM and the amount of PC added.The optimum process conditions of encapsulating PC with KGM determined by orthogonal optimization,that is,when 16%(w:v) KGM(particle size 50~90 μm) and 11%(w:v) PC were added into 10%(v:v) ethanol solution,the loading rate of PC was(14.75%±0.27%).At the same time,after digestion in the stomach and small intestine for 4 h,79.47% of PC in the KGM/PC microparticles was not released and reached the colon.To sum up,the encapsulated microparticles have great potential in colon-targeted delivery,which can provide a simple,effective,and safe encapsulation technology for industrial production.
作者
王朦朦
谢勇
蔡梦思
卫子颜
刘雄
WANG Mengmeng;XIE Yong;CAI Mengsi;WEI Ziyan;LIU Xiong(College of Food Science,Southwest University,Chongqing 400715,China)
出处
《食品工业科技》
CAS
北大核心
2022年第5期237-244,共8页
Science and Technology of Food Industry
关键词
魔芋葡甘聚糖
原花青素
靶向递送
微粒
包封
缓释
konjac glucomannan
procyanidins
targeted delivery
microparticles
encapsulation
sustained release