液相色谱串联质谱法同时测定调味酱中85种酸性合成色素

Simultaneous Determination of 85 Acid Synthetic Pigments in Sauce by Liquid Chromatography Tandem Mass Spectrometry

建立了测定调味酱中85种酸性合成色素的液相色谱串联质谱高通量筛查方法。样品经甲醇提取,C_(18)净化后,用水进行稀释,液相色谱串联质谱多反应监测模式测定,外标法定量。结果表明,85种酸性合成色素在10~1000 ng/mL的浓度范围内均呈良好的线性关系,相关系数均大于0.99,方法检出限为8.08~885.2μg/kg,定量限为26.94~2950.7μg/kg。在3个不同加标水平下,平均回收率在70.2%~116.5%,RSD为0.1%~15.0%。测定了市售的番茄酱、辣椒酱等50批次样品,在2批次样品中筛查出酸性红13、酸性橙II等禁止使用的色素。该方法通用性强、检测对象范围较广、分析时间短、灵敏度高,适合用于调味酱中85种酸性合成色素的分析测定。

A high-throughput liquid chromatography tandem mass spectrometry method was developed for the determination of 85 acid synthetic pigments in sauce.The acid synthetic pigments were extracted with methanol,purified with C_(18) and diluted with water.Subsequently,the sample was analyzed by liquid chromatography-tandem mass spectrometry under multiple reaction monitoring(MRM) mode,and quantified by external standard method.The results showed that the method had a good linearity in the range of 10~1000 ng/mL which correlation coefficients were greater than 0.99.The detection limit of the method was 8.08~885.2 μg/kg,and the quantitative limit was 26.94~2950.7 μg/kg.The average recoveries of synthetic pigments from spiked samples ranged from 70.2% to 116.5%,and the relative standard deviations were between 0.1% and 15.0%.This method was applied to analyze 50 batches of sauce such as tomato sauce and chili sauce,acid red 13 and acid orange II were screened out.The method has strong universality,wide range of detection objects,short analysis time and high sensitivity.It is suitable for the determination of 85 acid synthetic pigments in sauce.