鸡白痢、鸡伤寒沙门菌分子分型方法的验证

Verifi cation of Molecular Typing Methods for Salmonella Pullorum and Salmonella Gallinarum

为验证鸡白痢、鸡伤寒沙门菌分子分型方法的准确性,本研究以3株鸡白痢、鸡伤寒沙门菌国际参考菌株和306株国内分离株为研究对象,采用5种已知的分子分型方法开展验证性实验。结果发现,基于特定基因可变区的2种分型方法具有良好的特异性,其检测结果与生化分型结果一致,而3种基于特定基因单核苷酸多态性的分型方法则出现假阳性或假阴性的结果。上述结果表明,鸡白痢、鸡伤寒沙门菌分子分型方法的准确性仍需进一步验证。

The aim of this study was to verify the accuracy of molecular typing methods for Salmonella Pullorum and Salmonella Gallinarum.In this study,three international reference strains and 306 Chinese isolates of Salmonella Pullorum and Salmonella Gallinarum were used for verifying fi ve known molecular typing methods.The results showed that two genotyping methods based on variable regions of specifi c genes had good specifi city and their detection results were consistent with biochemical typing results while the other three genotyping methods based on single nucleotide polymorphism of specifi c genes produced false positive or false negative results.Therefore,the molecular typing methods for Salmonella Pullorum and Salmonella Gallinarum still needs to be further verifi ed for their accuracy.