特发性扩张型心肌病心衰相关基因和miRNA的筛选与分析

Screening and analyzing related genes and miRNA in heart failure caused by idiopathic dilated cardiomyopathy

目的:筛选特发性扩张型心肌病(idiopathic dilated cardiomyopathy,IDCM)心衰发展过程中潜在的关键基因和miRNA。方法:纳入GEO数据库中2个基因表达谱芯片,消除数据之间的批次效应并进行标准化后,筛选出共同差异表达基因(differentially expressed genes,DEGs),然后进行基因本体(gene ontology,GO)分析和KEGG富集分析,利用STRING构建蛋白质互作网络,利用miRNet获得靶向DEGs的miRNA,构建miRNA-DEGs网络,并用Cytoscape分析关键基因和miRNA。结果:根据|logFC|>0.4,P<0.05筛选出DEGs共210个,主要富集在PI3K信号通路、MAPK信号通路及与肿瘤相关的通路等。筛选出关键基因STAT3、MYC和CCND1等,以及关键miRNAs如miR-34a-5p、miR-17-5p和miR-20a-5p。结论:基于生物信息学方法对特发性扩张型心肌病心衰发展过程中潜在的关键基因和miRNA进行了筛选与分析,共筛选出210个差异基因及7个功能模块,分析了3个关键miRNAs,为进一步研究IDCM心衰的发生机制和治疗靶点提供思路。

Objective:To screen potential hub genes and miRNA in the process of heart failure caused by idiopathic dilated cardiomyopathy(IDCM). Methods:Two gene expression profiling chips from GEO database were included. After eliminating batch effects and normalizing the data,common differentially expressed genes(DEGs)were screened out. Then,gene ontology(GO)analysis and KEGG pathway enrichment analysis were performed. Protein-protein interaction(PPI)network was constructed by STRING online tool. miRNet was used to obtain miRNA targeting DEGs,and miRNA-DEGs network was constructed. And Cytoscape was used to analyze hub genes and miRNA. Results:According to |logFC| > 0.4 and P < 0.05,a total of 210 DEGs were screened out. These DEGs were mainly enriched in PI3 K signaling pathway,MAPK signaling pathway and tumor-related pathways. Hub genes including STAT3,MYC and CCND1 as well as key miRNAs such as miR-34 a-5 p,miR-17-5 p and miR-20 a-5 p were selected. Conclusion:Based on bioinformatics methods,the hub genes and miRNA in the process of heart failure caused by IDCM were screened and analyzed,a total of 210 differentially expressed genes and 7 functional modules were screened,and 3 key miRNAs were analyzed,which provided ideas for further research on the mechanism and therapeutic targets of IDCM heart failure.