斑马鱼条件位置偏爱模型在药物成瘾性研究中的应用与进展

Application and Progress of Conditioned Place Preference Using Zebrafish Model in Drug Addiction Research

斑马鱼(Danio rerio)具有体型小、易于饲养、繁殖率高、繁殖周期短、体外受精、胚胎透明易于观察等诸多优势,是药物成瘾研究当中理想的新型模式生物。本文概述药物成瘾问题,说明斑马鱼动物模型的体外发育、高通量、行为学特征丰富以及基因与人类具有相似性的优点,综述斑马鱼条件位置偏爱模型在各类常见成瘾性药物,包括甲基苯丙胺、尼古丁、可卡因、乙醇、咖啡因等中的应用与进展,指出该模型在毒品研究和管控领域进一步的发展趋势。

The zebrafish(Danio rerio)is an ideal model organism for drug addiction research because of its small size,ease of husbandry,high reproductive rate,short reproductive cycle,in vitro fertilization,and transparent embryos that can be easily observed.This paper firstly outlines the problem of drug addiction,and secondly analyses the characteristics of the zebrafish model,including in vitro development,high throughput,rich behavioural characteristics and genetic similarity to humans;and discusses the application and progress of the conditioned place preference using zebrafish model to various drug addiction studies,including methamphetamine,nicotine,cocaine,ethanol and caffeine.This study reviews the application and progress of the zebrafish model in drug addiction research and discusses its further development.