人激肽释放酶10、DJ-1、糖类抗原125、人附睾蛋白4在卵巢癌患者血清中的表达及其临床意义

Expression and significance of serum human kallikrein 10,DJ-1,carbohydrate antigen 125,human epididymis protein 4 in patients with ovarian cancer

目的探讨人激肽释放酶10(HK10)、DJ-1、糖类抗原125(CA125)及人附睾蛋白4(HE4)在卵巢癌患者血清中的表达及其临床意义。方法选取86例卵巢癌患者和50例卵巢良性病变患者,检测并比较卵巢癌和卵巢良性病变患者的血清HK10、DJ-1、CA125、HE4水平。比较不同临床特征卵巢癌患者的血清HK10、DJ-1、CA125、HE4水平,采用Pearson相关分析法分析卵巢癌患者血清HK10、DJ-1、CA125、HE4水平的相关性,采用受试者工作特征(ROC)曲线分析血清HK10、DJ-1、CA125、HE4单独及联合检测对卵巢癌的诊断价值。结果卵巢癌患者的血清HK10、DJ-1、CA125、HE4水平均明显高于卵巢良性病变患者,差异均有统计学意义(P﹤0.01)。国际妇产科联盟分期(FIGO)为Ⅲ~Ⅳ期卵巢癌患者的血清HK10、DJ-1、CA125、HE4水平均明显高于FIGO分期为Ⅰ~Ⅱ期的卵巢癌患者(P﹤0.01),低分化卵巢癌患者的血清HK10、DJ-1水平均明显高于中高分化卵巢癌患者(P<0.01)。卵巢癌患者的血清HK10和DJ-1水平呈正相关(r=0.648,P<0.05)。血清HK10、DJ-1、CA125、HE4单独及联合检测诊断卵巢癌的ROC曲线下面积分别为0.857、0.873、0.852、0.794、0.930,灵敏度分别为74.00%、76.00%、74.50%、71.20%、79.50%,特异度分别为70.00%、74.00%、69.50%、65.50%、76.00%。结论卵巢癌患者的血清HK10、DJ-1、CA125、HE4水平均较高,且与患者的临床特征有关。

Objective To investigate the expression and significance of serum human kallikrein 10(HK10),DJ-1,carbohydrate antigen 125(CA125)and human epididymis protein 4(HE4)in patients with ovarian cancer.Method A total of 86 patients with ovarian cancer and 50 patients with benign ovarian lesions were selected.The serum levels of HK10,DJ-1,CA125 and HE4 were detected and compared between patients with ovarian cancer and benign ovarian lesions.The serum levels of HK10,DJ-1,CA125 and HE4 in ovarian cancer patients with different clinical characteristics were compared.The correlation of serum HK10,DJ-1,CA125 and HE4 in ovarian cancer patients were analyzed by Pearson correlation method.Receiver operating characteristic(ROC)curve was plotted to analyze the value of serum HK10,DJ-1,CA125 and HE4 alone and combined detection in diagnose of ovarian cancer.Result The serum levels of HK10,DJ-1,CA125 and HE4 in patients with ovarian cancer were significantly higher than those in patients with benign ovarian lesions(P<0.01).The serum levels of HK10,DJ-1,CA125 and HE4 in ovarian cancer patients with International Federation of Gynecology and Obstetrics(FIGO)stageⅢ-Ⅳwere significantly higher than those with FIGO stageⅠ-Ⅱ(P<0.01).The serum levels of HK10 and DJ-1 in patients with poorly differentiated ovarian cancer were significantly higher than those with moderately and high differentiated ovarian cancer(P<0.01).There was a positive correlation between HK10 and DJ-1 in ovarian cancer patients(r=0.648,P<0.05).The area under ROC curve of serum HK10,DJ-1,CA125,HE4 alone and combined detection in diagnosis of ovarian cancer were 0.857,0.873,0.852,0.794 and 0.930,the sensitivity were 74.00%,76.00%,74.50%,71.20%and 79.50%,the specificity were 70.00%,74.00%,69.50%,65.50%and76.00%.Conclusion The serum levels of HK10,DJ-1,CA125 and HE4 in patients with ovarian cancer are increased,which are related to the clinical characteristics of patients.