重组酶聚合酶扩增技术在植物病毒检测中的应用

Applications of Recombinase Polymerase Amplification in Plant Virus Detection

随着分子生物学技术的发展,多种核酸等温扩增技术逐渐被开发出来。其中,重组酶聚合酶扩增(recombinase polymerase amplification,RPA)作为一种快速、灵敏的检测技术具有很大的优势。目前,RPA已应用于转基因生物、各类病原物及食品安全检测等多个领域,并作为新兴技术在植物病毒检测领域中快速发展。RPA技术只需一对引物,在恒温条件下(37-42℃)只需30 min左右即可完成反应,具有较高的灵敏度与特异性。因此,该技术正迅速成为一种能够用于条件有限的实验室或现场植物病毒检测的手段。本文介绍了RPA技术的检测原理、引物设计和应用方式,综述了其在植物病毒检测中的最新研究进展及存在的问题,为RPA技术在植物病毒检测中的应用提供参考。

With the development of molecular biology technology,a number of nucleic acid isothermal amplification technologies have been developed gradually.Recombinase polymerase amplification(RPA),as a rapid and sensitive detection technique,has great advantages.At present,RPA has been applied in many fields on detections,such as genetically modified organism,a variety of pathogens and food safety.As an emerging technology,RPA is developing rapidly in plant virus detection.The RPA technique requires only a pair of primers and is finished in only 30 min at constant temperature(37-42;C),which has high sensitivity and specificity.As a result,the RPA technique is rapidly becoming a very effective tool for detection of plant viruses in under-equipped laboratories or in on-site facilities.In this review,the detection principle,primer design and application for RPA are introduced,and the recent research progress and existing problems of RPA in plant virus detection is reviewed,aiming to provide references for the application of RPA in te detection of plant detection.