基于网络药理学探究络风宁2号方治疗慢性心力衰竭的作用机制

Exploring on the mechanism of Luo Fengning Ⅱ prescription in the treatment of chronic heart failure based on network pharmacology

目的基于网络药理学筛选出络风宁2号方主要活性化合物,预测络风宁2号方治疗慢性心力衰竭(心衰)的潜在作用靶点及信号通路,探讨其作用机制。方法利用TCMSP平台和相关文献记载检索络风宁2号方中10味药的化学成分和作用靶点;通过GeneCards数据库获取慢性心衰的相关靶标。采用David数据库对交集靶点进行GO功能富集分析和KEGG通路富集分析。结果络风宁2号方包含133个活性成分,对应1372个靶点。通过Venn图得出与慢性心衰的共同靶点97个,蛋白网络互作关键蛋白涉及白介素6(IL-6)、血管内皮生长因子A(VEGFA)、表皮生长因子受体(EGFR)等。通过GO分析得出1162条生物过程、45条细胞组分、106条分子功能,通过KEGG通路富集得出110条信号通路,其中通路主要涉及及PI3K-Akt信号通路、MAPK信号通路、HIF-1信号通路、Ras信号通路、IL-17信号通路等信号通路。结论本研究初步探索络风宁2号方治疗慢性心衰的作用机制,其活性化合物主要通过多靶点、多通路治疗慢性心衰,研究结果可为中医药治疗慢性心衰提供一定的理论基础与科学依据。

Objective The main active compounds of Luo FengningⅡwere screened out based on network pharmacology to predict the potential action targets and signaling pathways of Luo FengningⅡfor the treatment of chronic heart failure(HF)and to explore its mechanism of action.Methods The chemical constituents and action targets of 10 herbs in Luo FengningⅡwere retrieved using TCMSP platform and related literature records;the relevant targets for chronic heart failure were obtained through GeneCards database.Go functional enrichment analysis and KEGG pathway enrichment analysis of intersection targets were performed using David database.Results The Luo FengningⅡcontains 133 active ingredients,corresponding to 1372 targets.97 common targets with chronic HF were identified by the Venn diagram,and the protein network interaction key proteins involved interleukin 6(IL-6),vascular endothelial growth factor A(VEGFA),epidermal growth factor receptor(EGFR),and so on.1162 biological processes,45 cellular components,106 molecular functions through go analysis.And 110 signalling pathways were obtained by KEGG pathway enrichment,among which the pathways mainly involved PI3K Akt,MAPK,HIF-1,Ras,IL-17 and others.Conclusion In this paper,we initially explore the mechanism of action of Luo Fengning II for the treatment of chronic HF,and its active compounds mainly treat chronic HF through multi-target and multi-pathway,and the findings may provide a certain theoretical basis and scientific basis for the treatment of chronic HF in traditional Chinese medicine(TCM).