微小RNA-141和Yes相关蛋白1在胃癌组织中的表达及其对胃癌SGC-7901细胞增殖的影响

Expression of microRNA-141 and Yes-associated protein 1 in gastric cancer and their effects on proliferation of gastric cancer SGC-7901 cells

目的:探究微小RNA-141(miR-141)和Yes相关蛋白1(YAP1)在胃癌组织中的表达情况,同时分析它们对胃癌SGC-7901细胞增殖的影响。方法:选择行胃癌手术的40例患者为研究对象,取手术切除的胃癌组织及癌旁组织,采用荧光定量PCR(RT-qPCR)检测miR-141表达,采用免疫组化检测YAP1表达。分析miR-14、YAP1表达与胃癌患者临床病理特征的相关性。分别使用miR-141和YAP1对胃癌SGC-7901细胞系进行干预,分析它们对胃癌SGC-7901细胞增殖的影响。结果:胃癌组织中miR-141表达水平低于癌旁组织,YAP1蛋白表达水平高于癌旁组织(均P<0.05)。miR-141表达水平与胃癌患者组织分化程度及TNM分期存在明显相关性(均P<0.05)。YAP1表达水平与胃癌患者TNM分期、肿瘤直径以及组织分化程度相关(均P<0.05)。在24、48、72 h三个时间点,与阴性对照组比较,转染miR-141模拟物后,胃癌SGC-7901细胞活力出现明显降低,而转染miR-141抑制剂后胃癌SGC-7901细胞活力出现明显升高(均P<0.05)。在24、48、72 h三个时间点,与阴性对照组比较,转染YAP1沉默物siRNA YAP1的胃癌SGC-7901细胞活力出现明显降低,细胞迁移和侵袭能力明显下降(均P<0.05)。结论:胃癌组织中miR-141低表达,YAP1高表达,且两者表达水平同胃癌SGC-7901细胞活性密切相关,可以考虑将miR-141和YAP1作为胃癌治疗的新靶点。

Objective:To investigate the expression of microRNA-141(miR-141)and Yes-associated protein 1(Yap1)in gastric cancer,and to analyze their effects on the proliferation of gastric cancer SGC-7901 cells.Methods:40 patients undergoing gastric cancer surgery were selected as the research objects.The resected gastric cancer tissues and adjacent tissues were taken.The expression of miR-141 was detected by RT-qPCR,and the expression of YAP1 was detected by immunohistochemistry.The correlation between the expression of miR-14 and YAP1 and the clinicopathological features of patients with gastric cancer was analyzed.MiR-141 and Yap1 were used to intervene gastric cancer SGC-7901 cell line,and their effects on the proliferation of gastric cancer SGC-7901 cells were analyzed.Results:The expression level of miR-141 in gastric cancer was lower than that in adjacent tissues,and the expression level of YAP1 protein was higher than that in adjacent tissues(all P<0.05).The expression level of miR-141 was significantly correlated with the degree of tissue differentiation and TNM stage in patients with gastric cancer(all P<0.05).The expression level of YAP1 was correlated with TNM stage,tumor diameter and the degree of tissue differentiation in patients with gastric cancer(all P<0.05).At 24,48 and 72 hours,compared with the negative control group,the viability of gastric cancer SGC-7901 cells decreased significantly after transfection of miR-141 mimic,while the viability of gastric cancer SGC-7901 cells increased significantly after transfection of miR-141 inhibitor(all P<0.05).At 24,48 and 72 hours,compared with the negative control group,the viability of gastric cancer SGC-7901 cells transfected with YAP1 silencer siRNA YAP1 decreased significantly,and the ability of cell migration and invasion decreased significantly(all P<0.05).Conclusion:In gastric cancer tissue,the expression of miR-141 is low,and the expression of YAP1 is high,and their expression levels are closely related to the activity of gastric cancer SGC-7901 cells.MiR-141 and YAP1 can be considered as new targets for the treatment of gastric cancer.