结直肠癌患者血清循环肿瘤细胞、糖类抗原24-2、糖类抗原19-9水平及其与临床病理特征关系

Serum circulating tumor cells,carbohydrate antigen 24-2 and carbohydrate antigen 19-9 levels in patients with colorectal cancer and their relationship with clinicopathological features

目的:检测结直肠癌患者血清循环肿瘤细胞(CTC)、糖类抗原24-2(CA_(242))和糖类抗原19-9(CA_(199))水平,探讨它们与直肠癌患者不同临床病理特征的关系。方法:收集106例结直肠癌患者作为结直肠癌组,收集结直肠腺瘤患者100例作为结直肠腺瘤组,收集同期健康体检者100例作为对照组。采用实时荧光定量聚合酶链反应(qRT-PCR)检测CTC水平。采用全自动免疫分析仪检测CA_(242)和CA_(199)水平。比较三组研究对象CTC、CA_(242)、CA_(199)水平。比较不同临床病理特征结直肠癌患者CTC、CA_(242)、CA_(199)水平。分析CTC、CA_(242)、CA_(199)与结直肠癌患者不同临床病理特征的相关性。结果:结直肠癌组和结直肠腺瘤组患者CTC、CA_(242)、CA_(199)水平明显高于对照组,且结直肠癌组CTC、CA_(242)、CA_(199)水平明显高于结直肠腺瘤组(均P<0.05)。CTC、CA_(242)、CA_(199)水平在TNM分期为Ⅲ-Ⅳ期、分化程度为低分化、有淋巴结转移和肝转移的结直肠癌患者中明显升高(均P<0.05)。Spearman相关性分析结果显示,CTC、CA_(242)、CA_(199)水平与TNM分期、分化程度、淋巴结转移、肝转移密切相关(均P<0.05)。结论:CTC、CA_(242)、CA_(199)水平在结直肠癌患者中明显升高,且与结直肠癌患者临床病理特征密切相关。

Objective:To detect the levels of circulating tumor cell(CTC),carbohydrate antigen 24-2(CA_(242))and carbohydrate antigen 19-9(CA_(199))in serum of patients with colorectal cancer,and to explore their relationship with different clinicopathological features of patients with colorectal cancer.Methods:106 patients with colorectal cancer were collected as colorectal cancer group,100 patients with colorectal adenoma as colorectal adenoma group,and 100 healthy people in the same period as control group.The level of CTC was detected by qRT-PCR.The levels of CA_(242) and CA_(199) were detected by automatic immune analyzer.The levels of CTC,CA_(242) and CA_(199) in the three groups were compared.The levels of CTC,CA_(242) and CA_(199) in patients with colorectal cancer with different clinicopathological features were compared.The correlation between CTC,CA_(242),CA_(199) and different clinicopathological features of patients with colorectal cancer was analyzed.Results:The levels of CTC,CA_(242) and CA_(199) in colorectal cancer group and colorectal adenoma group were significantly higher than those in the control group,and the levels of CTC,CA_(242) and CA_(199) in colorectal cancer group were significantly higher than those in colorectal adenoma group(all P<0.05).The levels of CTC,CA_(242) and CA_(199) were significantly increased in colorectal cancer patients with TNM stageⅢ-Ⅳ,low differentiation,lymph node metastasis and liver metastasis(all P<0.05).Spearman correlation analysis showed that the levels of CTC,CA_(242) and CA_(199) were closely related to TNM stage,differentiation,lymph node metastasis and liver metastasis(all P<0.05).Conclusion:The levels of CTC,CA_(242) and CA_(199) are significantly increased in patients with colorectal cancer,and are closely related to the clinicopathological features.