蛹虫草整株粉、子实体粉和菌丝体粉的抗氧化性及其对经络体表电位差的影响

Antioxidant Properties of Powders of Cordyceps militaris Fungus,Sporophore and Mycelium and Their Effects on Body Surface Electrical Potential Difference Along Acupuncture Meridians

为研究蛹虫草整株粉、子实体粉和菌丝体粉的抗氧化性及其对经络体表电位的影响,测定了3种蛹虫草粉的DPPH自由基清除能力和氧自由基清除能力(ORAC)。召集40名健康志愿者按随机数字表法分为4组,空白组饮用100 mL蒸馏水,蛹虫草整株粉组、子实体粉组、菌丝体粉组各取相应粉末10 g冲泡于100 mL的蒸馏中饮用。选取志愿者同一条经络上的合穴和原穴作为经络体表电位的测量点,均以合穴为正极,原穴为负极,记录服用过程中经络体表电位的变化。蛹虫草整株粉、子实体粉和菌丝体粉的DPPH自由基清除能力值分别是(25.97±1.39)、(14.34±1.68)、(19.56±0.80)μmol/g(以Trolox质量计);ORAC值分别是(16.85±1.13)、(6.21±0.76)、(10.39±0.88)μmol/g(以Trolox质量计)。蛹虫草整株粉可以降低肺经、胃经、脾经、肾经、心包经、胆经和肝经的体表电位,提高小肠经的体表电位;子实体粉可以降低脾经和肾经的体表电位,提高小肠经的体表电位;菌丝体粉可以降低肾经和大肠经的体表电位,提高胃经、心包经和胆经的体表电位。抗氧化能力由高到低依次为蛹虫草整株粉、菌丝体粉和子实体粉,3种蛹虫草粉都能特异性的影响人体的经络体表电位。研究结果旨在为评价不同蛹虫草部位对人体的功效差异提供一定参考。

This study investigated the antioxidant properties and their effects on electrical potential difference(EPD)of body surface along acupuncture meridian of Cordyceps militaris fungus powder,sporophore powder and mycelium powder.The DPPH radical scavenging capacity and oxygen radical scavenging capacity(ORAC)of three kinds of Cordyceps militaris powders were determined.And forty healthy volunteers were divided into four groups equally according to the random number table.The control group drank 100 mL distilled water,and the Cordyceps militaris fungus powder group,sporophore powder group and mycelium powder group drank 100 mL of distilled water with 10 g corresponding powder.Data of the EPD between two acupoints in one acupuncture meridian were collected and recorded.The He acupoint was used as the positive pole,and the Yuan acupoint as the negative pole.The DPPH radical scavenging capacities of Cordyceps militaris fungus powder,sporophore powder and mycelium powder were(25.97±1.39)μmol/g,(14.34±1.68)μmol/g and(19.56±0.80)μmol/g.The ORAC values of Cordyceps militaris fungus powder,sporophore powder and mycelium powder were(16.85±1.13)μmol/g,(6.21±0.76)μmol/g and(10.39±0.88)μmol/g.Cordyceps militaris fungus powder decreased the EPDs of lung,stomach,spleen,kidney,pericardium,gallbladder and liver meridians,and increased the EPD of small intestine meridian.Cordyceps militaris sporophore powder decreased the EPDs of spleen and kindey meridians,and increased the EPD of small intestine meridian.Cordyceps militaris mycelium powder decreased the EPDs of kindey and large intestine meridians,and increased the EPDs of stomach,pericardium and gallbladder meridians.The antioxidant properties from the high to low were Cordyceps militaris fungus powder,mycelium powder and sporophore powder.These three kinds of Cordyceps militaris powders could specifically influence EPDs of acupuncture meridians.The results hope to provide some references for evaluating the efficacy of different parts of Cordyceps militaris.