克罗恩病组织中泛素特异性蛋白酶14的表达水平及意义

Expression level and significance of deubiquitinating enzyme USP14 in Crohn′s disease

目的探究克罗恩病组织中泛素特异性蛋白酶14(USP14)的表达水平及意义。方法选择2015年1月至2018年1月空军军医大学第一附属医院就诊的克罗恩病患者106例;采用蛋白免疫印记检测克罗恩病组织中USP14表达;克罗恩病简化内镜评分(SES-CD)评价疾病活动度;随访分析患者预后。结果USP14在克罗恩病活动度轻度中的表达低于其在中度、重度中的表达(P<0.05);中度中USP14水平较重度低(P<0.05)。克罗恩病患者USP14的表达水平与SEC-CD评分呈正相关关系(r=0.835,P<0.001)。106例患者中有42例(39.62%)预后不良,预后不良患者USP14水平(2.13±0.35)高于预后良好患者USP14水平(1.68±0.25),组间比较差异有统计学意义(P<0.05)。两组性别、年龄、病变部位、有无上消化道受累相比差异无统计学意义(P>0.05);两组疾病行为、肛周病变、USP14水平的表达相比差异有统计学意义(P<0.05)。非狭窄非穿透型与无肛周病变是影响克罗恩病患者预后的独立危险因素(P<0.05),USP14≤1.82是独立保护因素(P<0.05)。USP14诊断克罗恩病患者预后的灵敏度为59.38%、特异度为90.47%,AUC面积为0.785。结论USP14在克罗恩病中为高表达,其水平增加提示克罗恩病患者的病情加重、预后风险增加,是克罗恩病临床诊断的重要指标。

Objective To explore the expression level and significance of deubiquitinating enzyme USP14 in Crohn′s tissues.Methods A total of 106 patients with CROhn′s disease treated in our hospital(changed to a specific hospital)from January 2015 to January 2018 were selected.USP14 expression in CROhn′s disease tissues was detected by protein imprinting.Crohn′s disease simplified endoscopic score(SES-CD)was used to evaluate disease activity.Follow-up analysis of patient prognosis.Results The expression of USP14 in mild Crohn′s disease activity was lower than that in moderate and severe Crohn′s disease(P<0.05);The level of USP14 in moderate group was lower than that in severe group(P<0.05).The expression of USP14 was positively correlated with SEC-CD score in patients with Crohn′s disease(r=0.835,P<0.001).Of the 106 patients,42(39.62%)had poor prognosis,and the level of USP14 in poor prognosis patients(2.13±0.35)was higher than that of USP14(1.68±0.25),the difference between the two groups was statistically significant(t=7.638,P<0.05).There was no significant difference in gender,age,lesion location and upper gastrointestinal involvement between the two groups(P>0.05).There were significant differences in disease behavior,perianal lesions and USP14 expression between the two groups(P<0.05).Non stenotic,non penetrating and non perianal lesions were independent risk factors affecting the prognosis of patients with Crohn′s disease(P<0.05),and USP14≤1.82 was an independent protective factor(P<0.05).The sensitivity,specificity and AUC area of USP14 were 59.38%,90.47%and 0.785 respectively,which indicated that USP14 had a certain predictive value for the prognosis of patients with Crohn′s disease.Conclusion USP14 is highly expressed in Crohn′s disease,and the increase in its level indicates the aggravation of Crohn′s disease and the poor prognosis.It is an important indicator for the clinical diagnosis of Crohn′s disease.