绿色木霉缓解干旱胁迫对玉米幼苗根系生长的影响

Effects of Trichoderma viride Alleviating Drought Stress on Root Growth of Maize Seedlings

本试验以玉米品种郑单958为材料,用室内盆栽试验方式,设置正常水分和干旱两个处理,研究接种绿色木霉(Trichodermaviride)对干旱胁迫下玉米幼苗根系生长的影响。结果显示,正常水分条件下接种绿色木霉处理(TK),与CK相比,玉米幼苗的总根表面积、总投影面积、总根长分别增加41.73%、18.41%、10.12%,总根体积增加100.07%(P<0.05);根系构型中分支数、链接数、根链接平均长度与CK差异显著(P<0.05),交叉数、根尖数与CK相比差异不显著,分别增加5.93%、5.16%;比根长、比根表面积、分形维数、地上部鲜重、地下部鲜重与CK差异显著(P<0.05)。干旱胁迫下接种绿色木霉处理(DSTK),与DSCK相比,玉米幼苗的总根长、总根体积、总投影面积和总根表面积均显著提高(P<0.05);根系构型中分支数、链接数、根链接平均长度、根链接平均直径与DSCK差异显著(P<0.05);比根长、比根表面积、分形维数、地上部鲜重、地下部鲜重与DSCK差异显著(P<0.05),根冠比与DSCK相比差异不显著,增幅为25.28%。综上表明,干旱胁迫时,绿色木霉能够促进玉米幼苗根系形态指标、根系构型、生物量、根系几何特征指标的上升,从而缓解干旱胁迫对玉米幼苗根系的伤害。

To explore the effects of Trichoderma viride on root growth of maize seedlings under drought stress,the indoor pot experiment with two water treatments as control and drought stress was conducted with the cultivar Zhengdan 958 as experimental material.The indexes related to maize root growth were determined and analyzed.The results showed that under conditions of normal water and Trichoderma viride inoculation(TK),compared with no Trichoderma viride inoculation(CK),the total root surface area,total projected area and total root length of maize seedlings increased by 41.73%,18.41%and 10.12%,respectively,and the total root volume significantly increased by 100.07%(P<0.05);the number of root branches and links and the average length of root links were significantly different from those in CK(P<0.05),while the cross number and root tip number were not significantly different from those in CK,which increased by 5.93%and 5.16%,respectively;the specific root length,specific root surface area,fractal dimension,aboveground fresh weight and underground fresh weight were significantly different compared with CK(P<0.05).Under conditions of drought stress and Trichoderma viride inoculation(DSTK),compared with DSCK treatment,the total root length,total root volume,total projected area and total root surface area of maize seedlings increased significantly(P<0.05);the number of root branches and links,average length and diameter of root links in root architecture were significantly different from those in DSCK treatment(P<0.05);the specific root length,specific root surface area,fractal dimension,fresh weight of aboveground and underground were also significantly different compared with DSCK(P<0.05);the root-shoot ratio was not significantly different from that of DSCK group with an increase of 25.28%.In conclusion,the inoculation of Trichoderma viride could promote the growth of maize root morphological index,root architecture,biomass and root geometric indexes under drought stress,so as to alleviate the damage of drought stress on maize root system。