白首乌酵素对癫痫小鼠认知损伤影响研究

Effect of Cynanchum auriculatum ferment on cognitive impairment in epileptic mice

为研究白首乌酵素对癫痫小鼠认知损伤缓解作用,以江苏滨海白首乌为原料制备白首乌酵素。建立癫痫小鼠模型,分别测定无特定病原体(SPF)小鼠认知功能,血清和海马体组织的超氧化物歧化酶(SOD)、丙二醛(MDA)水平和海马体组织的肿瘤坏死因子-α(TNF-α)、白细胞介素1β(IL-1β)水平和核因子-κB(NF-κB)P65及其抑制蛋白(IκB)表达水平。结果表明,酵素中的二苯乙烯苷含量为78.18μg/mL;添加酵素可显著缩短逃避潜伏期(57.7%)、增加跨越平台次数(83.3%)(P<0.05);组织切片显示海马体组织细胞分布轮廓较清晰,顶状树突较明显;血清、海马体组织的SOD水平分别提高19.9%、33.3%,MDA水平分别降低16.0%、26.4%;NF-α、IL-1β分别降低27.8%、20.6%;(p-NF-κB)P65、(p-IκB)蛋白表达水平显著降低至121.6%、119.1%(P<0.05)。表明白首乌酵素可能通过NF-κB信号通路缓解癫痫小鼠认知损伤。

In order to study the alleviating effect of Cynanchum auriculatum ferment on cognitive impairment in epileptic mice,C.auriculatum ferment was prepared using Jiangsu C.auriculatum as raw material.An epilepsy mouse model was established,and the cognitive function,superoxide dismutase(SOD),malondialdehyde(MDA)level of serum and hippocampal tissue,and the levels of tumor necrosis factor-α(TNF-α),interleukin 1β(IL-1β)and the expression level of nuclear factor-κB(NF-κB)P65 and its inhibitor protein(IκB)of hippocampus tissues were measured,respectively.The results showed that the content of stilbene glycoside was 78.18μg/ml.The addition of the ferment could significantly shorten the escape latency(57.7%)and increase the number of platform crossings(83.3%)(P<0.05).The tissue sections showed that the distribution of hippocampus cells was clear and the apical dendrites were obvious.SOD levels in serum and hippocampus increased by 19.9%and 33.3%,respectively,while MDA levels decreased by 16.0%and 26.4%,respectively.NF-αand IL-1βdecreased by 27.8%and 20.6%,respectively.(p-NF-κB)P65,(p-IκB)protein expression levels significantly reduced to 121.6%and 119.1%,respectively(P<0.05).These results suggest that C.auriculatum ferment may alleviate cognitive impairment in epileptic mice through the NF-κB signaling pathway.