细胞穿透肽增强氨基糖苷类抗生素杀菌作用的方法

Method of Enhancing the Bactericidal Action of Aminoglycoside Antibiotics by Cell Penetrating Peptides

为验证细胞穿透肽能否增强氨基糖苷类抗生素杀菌作用,选用具代表性的3种氨基糖苷类抗生素(妥布霉素、卡那霉素及链霉素)处理平台期大肠杆菌。在特定抗生素处理浓度和处理时间下,进行平台期大肠杆菌杀伤效果组间比较,用ANOVA算法分析试验结果差异性;并使用NPN染色法探究细胞穿透肽增强氨基糖苷类抗生素杀菌作用机制。结果表明:细胞穿透肽预处理可以极显著增强妥布霉素、卡那霉素及链霉素杀灭平台期大肠杆菌,且细胞穿透肽对大肠杆菌细胞外膜造成轻微破坏。

In order to verify whether the cell penetrating peptides could enhance the bactericidal action of aminoglycoside antibiotics,three representative aminoglycoside antibiotics(tobramycin,kanamycin and streptomycin)were selected to treat Escherichia coli at plateau phase.Under the specific antibiotic treatment concentration and treatment time,the killing effect of Escherichia coli at plateau phase was compared between groups,and the differences of experimental results was analyzed by using the ANOVA algorithm.Then,the NPN staining method was used to explore the mechanism of cell penetrating peptides enhancing the bactericidal action of aminoglycoside antibiotics.The results showed that the pretreatment with cell penetrating peptides could extremely significantly enhance tobramycin,kanamycin and streptomycin to kill Escherichia coli at plateau phase,and the cell penetrating peptides could slightly damage the cell outer membrane of Escherichia coli.